Temperature effects on molecular processes which lead to stage differentiation in Leishmania.
Temperature effects on molecular processes which lead to stage differentiation in Leishmania.
复制标题
温度对导致利什曼原虫阶段分化的分子过程的影响。
DOI：
10.1002/j.1460-2075.1988.tb03147.x
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
C. Jaffe
中科院分区：
文献类型：
--
作者：
M. Shapira;J. Mcewen;C. Jaffe
我们研究了生长阶段和温度对利什曼原虫阶段分化过程中发生的形态和分子过程的影响。寄生虫不同的能力，它们的转化axenically进行了比较。在无菌转化的菌株中观察到典型的热休克反应。70和83 kd的热休克蛋白（HSP）的转录和合成沿着细胞蛋白质合成的减少，包括微管蛋白。微管蛋白基因转录的变化也被注意到。在培养物中不转化的菌株中，热休克蛋白被诱导，但细胞蛋白质合成不受影响，并且在微管蛋白转录和表达中没有观察到差异。HSP 70和83在无鞭毛体中保持组成型表达。HSP 83转录水平沿着前鞭毛体生长曲线增加。β-微管蛋白的转录也受到生长阶段和温度升高的影响，尽管α-微管蛋白保持不变。在热休克的前鞭毛体中诱导了一种无鞭毛体样杂交模式，其中β微管蛋白的较大转录物（2.8 kb）占主导地位，而前鞭毛体转录物（2.4 kb）减少。微管蛋白的表达是敏感的温度控制在墨西哥利什曼原虫亚马逊，虽然没有直接相关的HSP表达。
We have studied the effects of growth phase and temperature on the morphological and molecular processes that occur during stage differentiation of Leishmania. Parasites which differ in their ability to transform axenically were compared. A typical heat shock response is observed in strains that transform axenically. Heat shock proteins (HSPs) of 70 and 83 kd are transcribed and synthesized along with a decrease in cellular protein synthesis, including tubulin. Changes in the transcription of the tubulin gene are also noted. In strains which do not transform in culture HSPs are induced, but cellular protein synthesis is unaffected and no differences are observed in tubulin transcription and expression. HSPs 70 and 83 remain constitutively expressed in amastigotes. HSP 83 transcription increases along the promastigote growth curve. beta‐Tubulin transcription is also affected by growth phase and temperature increase, though alpha‐tubulin remains unaltered. An amastigote‐like hybridization pattern is induced in heat‐shocked promastigotes, in which a larger transcript for beta‐tubulin (2.8 kb) becomes dominant and the promastigote transcript (2.4 kb) decreases. Tubulin expression is susceptible to temperature control in Leishmania mexicana amazonensis, though direct correlation to HSP expression was not demonstrated.