The measurement of acid and alkaline phosphatase in gingival crevicular fluid during orthodontic tooth movement
The measurement of acid and alkaline phosphatase in gingival crevicular fluid during orthodontic tooth movement
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DOI：
10.1016/s0889-5406(96)70152-x
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发表时间：
1996-03-01
影响因子：
3
通讯作者：
Keeling, SD
中科院分区：
文献类型：
--
作者：
Insoft, M;King, GJ;Keeling, SD
这项研究检查了龈沟液（GCF）中的酸性和碱性磷酸酶活性，以了解是否可以在正畸牙齿移动期间监测人类受试者的骨转换动态。纵向观察三名女性受试者以评估牙齿移动、牙菌斑和炎症。对于每个受试者，随机选择一颗前磨牙作为对照，不进行治疗，另一颗前磨牙用 100 克的力向颊侧移动。每周收集 GCF 并检测磷酸酶。碱性磷酸酶在第一周和第三周之间达到峰值，随后酸性磷酸酶在第三周和第六周之间增加。第一周后，牙齿平均移动 0.9 毫米。在接下来的 3 周内，发生额外 0.9 毫米的移动，随后在第 4 至 6 周发生 1.4 毫米的移动。另外 30 名患者被随机分为头带/咬合板组、生物器组和对照组，并在上颌第一磨牙处进行了横截面采样。 GCF 磷酸酶活性根据牙齿上的位置、治疗方式、治疗持续时间、牙龈炎症和牙菌斑积累的函数进行评估。在治疗组中，斑块指数与内侧或远端的酸性或碱性磷酸酶活性没有关系。然而，在治疗组中，随着远端炎症的发生，碱性磷酸酶增加，而治疗组中，内侧的酸性磷酸酶始终高于远端。在治疗牙齿的 GCF 中发现酸性和碱性磷酸酶的交替峰，作为治疗持续时间的函数。这些变化的顺序与啮齿动物正畸牙齿移动模型中牙槽骨转换的报道相似。我们的结论是，GCF 中的磷酸酶活性可能是监测组织对正畸治疗反应的有用手段。
This study examines acid and alkaline phosphatase activities in gingival crevicular fluid (GCF) to learn whether bone turnover dynamics can be monitored in human subjects during orthodontic tooth movement. Three female subjects were observed longitudinally to assess tooth movement, plaque, and inflammation. For each subject, one randomly selected premolar served as the control and was not treated, and another was moved buccally with 100 gm of force. The GCF was collected weekly and assayed for phosphatases. Alkaline phosphatase peaked between the first and third weeks, followed by an increase in acid phosphatase between the third and sixth weeks. After the first week, tooth movement averaged 0.9 mm. An additional 0.9 mm of movement occurred during the next 3 weeks, followed by 1.4 mm during weeks 4 to 6. Thirty additional patients, randomly divided into headgear/biteplate, bionator, and control groups, were also sampled cross-sectionally at the maxillary first molars. The GCF phosphatase activities were assessed as functions of location on the tooth, treatment modality, duration of treatment, gingival inflammation, and plaque accumulation. The plaque index did not show a relationship to either acid or alkaline phosphatase activity on the mesial or distal in the treated groups. However, alkaline phosphatase increased with inflammation on the distal in treated groups and acid phosphatase was consistently higher on the mesial than on the distal in the treatment groups. Alternating peaks of acid and alkaline phosphatase were found in GCF of treated teeth as functions of treatment duration. The sequence of these changes is similar to that reported for alveolar bone turnover in a rodent orthodontic tooth movement model. We conclude that phosphatase activities in GCF may be a useful means for monitoring tissue responses to orthodontic treatment.