Multiple thiol-anchor capped DNA-gold nanoparticle conjugates
Multiple thiol-anchor capped DNA-gold nanoparticle conjugates
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DOI：
10.1093/nar/30.7.1558
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发表时间：
2002-04-01
影响因子：
14.9
通讯作者：
Letsinger, RL
中科院分区：
文献类型：
--
作者：
Li, Z;Jin, RC;Letsinger, RL
我们报道了一种新的三巯基加帽的寡脱氧核糖核苷酸和金纳米粒子的共轭物的合成。这些DNA-金纳米粒子共轭物表现出比单巯基和环状二硫键加帽的寡脱氧核糖核苷酸制备的类似物更高的稳定性，但可比较的杂交性能。提供了一系列条件下的稳定性的定量分析。值得注意的是，这种新的三硫醇寡脱氧核糖核苷酸可用于稳定直径>30 nm的颗粒，这对于许多诊断应用是必不可少的。
We report the synthesis of a novel trithiol-capped oligodeoxyribonucleotide and gold nanoparticle conjugates prepared from it. These DNA-gold nanoparticle conjugates exhibit substantially higher stability than analogs prepared from monothiol and cyclic disulfide-capped oligodeoxyribonucleotides, but comparable hybridization properties. A quantitative analysis of their stability under a range of conditions is provided. Significantly, this novel trithiol oligodeoxyribonucleotide can be used to stabilize particles >30 nm in diameter, which are essential for many diagnostic applications.