Supported Lipid Bilayers for Atomic Force Microscopy Studies
Supported Lipid Bilayers for Atomic Force Microscopy Studies
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DOI：
10.1007/978-1-4939-8591-3_8
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发表时间：
2018-01-01
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影响因子：
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通讯作者：
Lyubchenko, Yuri L.
中科院分区：
文献类型：
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作者：
Lv, Zhengjian;Banerjee, Siddhartha;Lyubchenko, Yuri L.
纳米成像方法，特别是原子力显微镜 (AFM)，广泛用于研究生物分子与支撑的脂质双层 (SLB) 的相互作用，而脂质双层本身就是细胞膜的传统模型。这些研究的成功取决于在所需的观察期内是否有稳定的 SLB，该观察期可能会延长几个小时。 AFM 的应用要求 SLB 具有光滑的形态，从而能够对其表面的蛋白质和其他分子进行可视化。在此，我们描述了在云母支持上使用 1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱 (POPC) 和 1-棕榈酰-2-油酰-sn-甘油-3-磷酸-L-丝氨酸 (POPS) 进行 SLB 组装的协议。我们的方法使我们能够组装无缺陷的 POPC 和 POPS SLB，并保持稳定至少 8 小时。通过使用延时 AFM 监测淀粉样蛋白的表面聚集情况，说明了这种光滑稳定表面的应用。
Nanoimaging methods, atomic force microscopy (AFM) in particular, are widely used to study the interaction of biological molecules with the supported lipid bilayer (SLB), which itself is a traditional model for cellular membranes. Success in these studies is based on the availability of a stable SLB for the required observation period, which can extend several hours. The application of AFM requires that the SLB have a smooth morphology, thus enabling visualization of proteins and other molecules on its surface. Herein, we describe protocols for SLB assembly by using 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (POPS) on a mica support. Our methodology enables us to assemble defect-free POPC and POPS SLBs that remain stable for at least 8 h. The application of such smooth and stable surfaces is illustrated by monitoring of the on-surface aggregation of amyloid proteins with the use of time-lapse AFM.