An overview of gibberellin metabolism enzyme genes and their related mutants in rice
An overview of gibberellin metabolism enzyme genes and their related mutants in rice
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DOI：
10.1104/pp.103.033696
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发表时间：
2004-04-01
期刊：
影响因子：
7.4
通讯作者：
Matsuoka, M
中科院分区：
文献类型：
--
作者：
Sakamoto, T;Miura, K;Matsuoka, M
为了加深对水稻赤霉素代谢的了解，（Oryza sativa）中，我们使用所有可用的水稻DNA数据库集中筛选并鉴定了编码以下GA代谢酶的29个候选基因：对映-柯巴基二磷酸合酶（CPS），对映-贝壳杉烯合酶（KS），对映-贝壳杉烯氧化酶（KC），对映-贝壳杉烯酸氧化酶（KAO），GA 20-氧化酶（GA 20 ox），GA 3-氧化酶（GA 3 ox），和GA 2-氧化酶（GA 2 ox）。与拟南芥基因组相比，在水稻基因组中鉴定出多个CPS样、KS样和KO样基因，其中大多数是连续排列的。我们还确定了18个GA缺乏水稻突变体在6个不同的位点从水稻突变体集合。基于突变体和表达分析，我们证明了催化GA生物合成途径中的早期步骤的酶（即CPS，KS，KO和KAO）主要由单基因编码，而用于后期步骤的酶（即GA 20 ox，GA 3 ox和GA 2 ox）由基因家族编码。剩余的CPS-样，KS-样，和KO-样基因可能参与二萜植物抗毒素的生物合成，而不是GA，因为两个CPS-样和三个KS-样基因（OsCPS 2，OsCPS 4，OsKS 4，OsKS 7和OsKS 8）的表达增加的UV照射，和这些基因（OsCPS 2，OsCPS 4，OsKS 4，和OsKS 7）中的四个也诱导诱导处理。
To enhance our understanding of GA metabolism in rice (Oryza sativa), we intensively screened and identified 29 candidate genes encoding the following GA metabolic enzymes using all available rice DNA databases: ent-copalyl diphosphate synthase (CPS), ent-kaurene synthase (KS), ent-kaurene oxidase (KC), ent-kaurenoic acid oxidase (KAO), GA 20-oxidase (GA20ox), GA 3-oxidase (GA3ox), and GA 2-oxidase (GA2ox). In contrast to the Arabidopsis genome, multiple CPS-like, KS-like, and KO-like genes were identified in the rice genome, most of which are contiguously arranged. We also identified 18 GA-deficient rice mutants at six different loci from rice mutant collections. Based on the mutant and expression analyses, we demonstrated that the enzymes catalyzing the early steps in the GA biosynthetic pathway (i.e. CPS, KS, KO, and KAO) are mainly encoded by single genes, while those for later steps (i.e. GA20ox, GA3ox, and GA2ox) are encoded by gene families. The remaining CPS-like, KS-like, and KO-like genes were likely to be involved in the biosynthesis of diterpene phytoalexins rather than GAs because the expression of two CPS-like and three KS-like genes (OsCPS2, OsCPS4, OsKS4, OsKS7, and OsKS8) were increased by UV irradiation, and four of these genes (OsCPS2, OsCPS4, OsKS4, and OsKS7) were also induced by an elicitor treatment.