Culturable endophytic bacterial flora of the maturing leaves and roots of rice plants (Oryza sativa) cultivated in a paddy field
Culturable endophytic bacterial flora of the maturing leaves and roots of rice plants (Oryza sativa) cultivated in a paddy field
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DOI：
10.1264/jsme2.22.175
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发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Morisaki, Hisao
中科院分区：
文献类型：
--
作者：
Mano, Hironobu;Tanaka, Fumiko;Morisaki, Hisao
研究了水稻成熟过程中叶和根中的可培养内生细菌，并与先前研究中报道的水稻种子中的细菌进行了比较。从叶片中分离出可培养的附生细菌，并与内生细菌进行了比较。根据16S rRNA基因序列对分离株进行鉴定。从叶片中分离到与芽孢杆菌属、短杆菌属、甲基杆菌属、鞘氨醇单胞菌属和泛菌属密切相关的内生菌，从根中分离到与芽孢杆菌属、短杆菌属、分枝杆菌属、肠杆菌属和金黄杆菌属密切相关的内生菌。叶片内生细菌植物群与种子相似，但与根不同。随着水稻植株的成熟，叶片中革兰氏阴性菌的比例逐渐增加（与短小杆菌属相关的菌株数量减少，而与甲基杆菌属和泛菌属相关的菌株数量增加），而根中革兰氏阴性菌株的比例减少（与根瘤菌目相关的菌株数量减少，并被与能够形成内生孢子的芽孢杆菌属和短芽孢杆菌属相关的菌株所取代）。叶内和叶表分离到的菌株分别与13种和19种有密切关系。其中，短小芽孢杆菌、柠檬酸短杆菌（Curtobacterium citreum）或C. flavonifaciens、C. pusillum作为最接近的物种），水生甲基杆菌、藤泽甲基杆菌（或耐辐射甲基杆菌）、甜瓜鞘氨醇单胞菌和矢渊鞘氨醇单胞菌在两个地点都是常见的，并且在两个地点都占大多数。
The culturable endophytic bacteria in the leaves and roots of rice plants (Oryza sativa, cultivar Kinuhikari) cultivated on an experimental plot adjacent to a paddy field were studied during the maturation process, and a comparison with the bacteria inside rice seeds reported in a previous study was conducted. Culturable epiphytic bacteria were also isolated from the leaves and compared with the endophytic bacteria. The isolates were identified based on their 16S rRNA gene sequences. Many endophytes closely related to Bacillus, Curtobacterium, Methylobacterium, Sphingomonas and Pantoea from the leaves, and to Bacillus, Brevibacillus, Mycobacterium, Enterobacter, and Chryseobacterium from the roots were isolated. The endophytic bacterial flora of the leaves was similar to that of the seeds, but differed from that of the roots. As the rice plant matured, the ratio of Gram-negative strains among the isolates increased in the leaves (the isolates related to Curtobacterium decreased in number and those related to Methylobacterium and Pantoea increased), whereas the ratio of Gram-negative strains decreased in the roots (the isolates related to the order Rhizobiales decreased in number and were replaced by strains related to Bacillus and Brevibacillus capable of forming endospores). The isolates from the inside and surface of the leaf were closely related to 13 and 19 species, respectively. Among these isolates, the following 6 species related to Bacillus pumilus, Curtobacterium citreum (or C. flaccumfaciens, C. pusillum as the closest species), Methylobacterium aquaticum, Methylobacterium fujisawaense (or M radiotolerans), Sphingomonas melonis and Sphingomonas yabuuchiae were common to both sites and constituted the majority at both sites.