Effects of osmotic pressure, glucose, and temperature on neurons in preoptic tissue slices.
Effects of osmotic pressure, glucose, and temperature on neurons in preoptic tissue slices.
复制标题
渗透压、葡萄糖和温度对视前组织切片中神经元的影响。
DOI：
10.1152/ajpregu.1984.247.2.r335
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Boulant,JA
中科院分区：
文献类型：
--
作者：
Silva,NL;Boulant,JA
视前区含有温度敏感神经元、渗透敏感神经元和葡萄糖敏感神经元，它们分别参与温度、渗透压和代谢调节。在这项研究中，大鼠视前区组织切片被用来检查这些神经元的特异性，通过确定他们的个人放电率响应热，渗透压，和葡萄糖刺激。在根据温度敏感性分类的神经元中，35%是温敏感的，10%是冷敏感的，55%是温度不敏感的。其中，107个神经元也进行了测试，他们的组织切片灌注低葡萄糖和/或高渗介质的反应。这些实验性灌注影响了三分之一的温度不敏感神经元和近一半的热敏神经元，并且通常兴奋温敏神经元和抑制冷敏神经元。由于大多数反应性神经元同时受到高渗和低葡萄糖介质的影响，因此在对葡萄糖敏感的神经元和对葡萄糖敏感的神经元之间也观察到缺乏特异性。虽然这些发现并不表明视前神经元有很强的功能特异性，但它们确实支持了以前强调调节系统之间相互作用的研究。
The preoptic area contains thermosensitive, osmosensitive, and glucosensitive neurons, which have been implicated in thermal, osmotic, and metabolite regulations, respectively. In this study, rat preoptic tissue slices are used to examine the specificity of these neurons by determining their individual firing rate responses to thermal, osmotic, and glucose stimuli. Of the neurons classified according to thermosensitivity, 35% were warm sensitive, 10% were cold sensitive, and 55% were temperature insensitive. Of these, 107 neurons were also tested for their responses to tissue slice perfusions of low-glucose and/or hyperosmotic media. These experimental perfusions affected one-third of the temperature-insensitive neurons and nearly half of the thermosensitive neurons and generally excited warm-sensitive neurons and inhibited cold-sensitive neurons. A lack of specificity was also observed between the groups of osmosensitive and glucosensitive neurons, since most of the responsive neurons were affected by both hyperosmotic and low-glucose media. Although these findings do not suggest a strong functional specificity for preoptic neurons, they do support previous studies that emphasize interactions between regulatory systems.