Differential Expression of Distinct Members of Rho Family GTP-Binding Proteins during Neuronal Development: Identification ofRac1B, a New Neural-Specific Member of the Family
Differential Expression of Distinct Members of Rho Family GTP-Binding Proteins during Neuronal Development: Identification ofRac1B, a New Neural-Specific Member of the Family
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Rho 家族 GTP 结合蛋白不同成员在神经元发育过程中的差异表达：Rac1B（该家族新的神经特异性成员）的鉴定
作者：
M. Malosio;D. Gilardelli;Simona Paris;C. Albertinazzi;I. de Curtis
以前的研究表明，Rho家族的小GTP结合蛋白参与了细胞肌动蛋白细胞骨架的组织。这些GTP酶在脊椎动物发育过程中的功能尚不清楚。为了了解这些蛋白在神经元发育过程中可能的作用，我们克隆并测序了5个在发育中的鸡神经视网膜细胞中表达的成员。我们已经鉴定了4个鸡基因cRhoA、cRhoB、cRhoC和cRac 1A，它们与已知的人类基因同源，还鉴定了一个新的Rac基因cRac 1B。四个确定的转录本在鸡胚胎中的分布的分析表明，第一次高水平的表达Rho家族基因在脊椎动物发育中的神经系统，不同的转录本的分布模式不同。特别是，cRhoA和cRac 1A基因表达似乎普遍存在于整个胚胎中，cRhoB转录本在活跃延伸神经突的神经元群体中更为突出，而新发现的cRac 1B基因仅在发育中的神经系统中均匀表达。对这五个基因表达的时间分析表明与发育中的视网膜和视网膜顶盖通路内发生的形态发生事件相关。表位标记的cRac 1B在视网膜神经元中的表达显示该蛋白在细胞体中和沿着神经突弥散分布。作为一个整体，我们的研究结果表明，重要的作用，无处不在的和神经特异性的Rho家族成员在收购成熟的神经元表型。
Previous studies on small GTP-binding proteins of the Rho family have revealed their involvement in the organization of cell actin cytoskeleton. The function of these GTPases during vertebrate development is not known. With the aim of understanding the possible role of these proteins during neuronal development, we have cloned and sequenced five members expressed in developing chick neural retinal cells. We have identified four chicken genes, cRhoA, cRhoB, cRhoC, and cRac1A, homologous to known human genes, and a novel Rac gene,cRac1B. Analysis of the distribution of four of the identified transcripts in chicken embryos shows for the first time high levels of expression of Rho family genes in the vertebrate developing nervous system, with distinct patterns of distribution for the different transcripts. In particular, cRhoA andcRac1A gene expression appeared ubiquitous in the whole embryo, and the cRhoB transcript was more prominent in populations of neurons actively extending neurites, whereas the newly identified cRac1B gene was homogeneously expressed only in the developing nervous system. Temporal analysis of the expression of the five genes suggests a correlation with the morphogenetic events occurring within the developing retina and the retinotectal pathway. Expression of an epitope-tagged cRac1B in retinal neurons showed a diffuse distribution of the protein in the cell body and along neurites. Taken as a whole, our results suggest important roles for ubiquitous and neural-specific members of the Rho family in the acquisition of the mature neuronal phenotype.
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影响因子：
--
作者：
G. Pfeifer;A. Riggs
通讯作者：
G. Pfeifer;A. Riggs
层粘连蛋白受体。
DOI：
10.1146/annurev.cb.07.110191.000443
发表时间：
1991
期刊：
Annual review of cell biology
影响因子：
--
作者：
Mecham,RP
通讯作者：
Mecham,RP
影响因子：
4.1
作者：
Gibson, RM;Wilson-Delfosse, AL
通讯作者：
Wilson-Delfosse, AL
磷酸酪氨酸修饰的蛋白质位于小鸡神经视网膜的穆勒细胞中。
影响因子：
3.4
作者：
Biscardi,JS;Cooper,NG;Maness,PF
通讯作者：
Maness,PF
层粘连蛋白受体 α 6 β 1 及其亚型在发育中鸡视网膜中的功能和空间分布。
DOI：
10.1242/dev.118.2.377
发表时间：
1993
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
deCurtis,I;Reichardt,LF
通讯作者：
Reichardt,LF