Picrosirius Red Staining: A Useful Tool to Appraise Collagen Networks in Normal and Pathological Tissues
Picrosirius Red Staining: A Useful Tool to Appraise Collagen Networks in Normal and Pathological Tissues
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DOI：
10.1369/0022155414545787
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发表时间：
2014-10-01
影响因子：
3.2
通讯作者：
Changotade, Sylvie
中科院分区：
文献类型：
--
作者：
Lattouf, Raed;Younes, Ronald;Changotade, Sylvie
细胞外基质成分的特异性染色特别有助于研究组织重塑，特别是在结缔组织病理学的情况下。正如Junqueira及其同事在1979年开发的那样，苦味酸天狼星红的特异性染色是研究不同组织中胶原蛋白网络的最重要的染色剂之一。在偏振光下，胶原束呈现绿色、红色或黄色，并且容易与黑色背景区分开，从而允许进行定量形态测定分析。正如Junqueira及其同事指出的那样，许多研究使用彩色染色来区分胶原束并指定胶原类型，但其他研究报告偏振颜色仅反映纤维厚度和包装。使用一个简单的组织学例子，我们的研究说明了苦味酸天狼星红染色无法区分胶原类型，因为这种染料吸收的偏振光量严格取决于胶原束的方向。
Specific staining of the extracellular matrix components is especially helpful in studying tissue remodeling, particularly in the case of connective tissue pathologies. As developed by Junqueira and colleagues in 1979, specific staining by Picrosirius red is one of the most important stains to study collagen networks in different tissues. Under polarized light, collagen bundles appear green, red or yellow, and are easily differentiated from the black background, thus allowing for quantitative morphometric analysis. As Junqueira and colleagues point out, many studies use color staining to differentiate collagen bundles and to specify collagen types, yet other studies report that polarized colors only reflect fiber thickness and packing. Using a simple histological example, our study illustrates the inability of Picrosirius red staining to differentiate collagen types, since the absorbed amount of polarized light by this dye strictly depends on the orientation of the collagen bundles.