Comparison of Foxp3+ Regulatory T cells and CD163+ Macrophages in Invasive and Non-invasive Extramammary Paget's Disease
Comparison of Foxp3+ Regulatory T cells and CD163+ Macrophages in Invasive and Non-invasive Extramammary Paget's Disease
复制标题
侵袭性与非侵袭性乳房外Paget病中Foxp3阳性调节性T细胞与CD163阳性巨噬细胞的比较
DOI：
10.2340/00015555-1453
复制
发表时间：
2012-01-01
影响因子：
3.6
通讯作者：
Aiba, Setsuya
中科院分区：
文献类型：
--
作者：
Fujimura, Taku;Kambayashi, Yumi;Aiba, Setsuya
通过表达 CD4、CD25 和 Foxp3 来识别的调节性 T 细胞 (Treg) 与免疫抑制性巨噬细胞（例如 CD163(+) M2 巨噬细胞）一起参与维持外周耐受。本研究的目的是阐明 Tregs 和 CD163(+) 巨噬细胞在侵袭性和非侵袭性乳房外佩吉特病中的参与。对10名非侵袭性乳房外Paget病患者和7名侵袭性乳房外Paget病患者的病变皮肤固定切片进行免疫组织学检查，检测CD4(+)CD25(+)Foxp3+ Tregs、CD163(+) M2巨噬细胞和基质金属蛋白酶-9(+)细胞的存在。在非侵袭性乳房外佩吉特病中观察到的 CD4(+)CD25(+)Foxp3(+) Tregs 数量少于侵袭性乳房外佩吉特病。相比之下，仅在侵袭性乳房外佩吉特病中检测到较高数量的CD163(+)巨噬细胞和金属蛋白酶-9(+)细胞。这些发现表明，乳房外佩吉特病中免疫抑制细胞的诱导因肿瘤阶段而异。
Regulatory T cells (Tregs), identified by the expression of CD4, CD25 and Foxp3, together with immunosuppressive macrophages, such as CD163(+) M2 macrophages, are involved in maintaining peripheral tolerance. The aim of this study was to elucidate the involvement of Tregs and CD163(+) macrophages in invasive and non-invasive extramammary Paget's disease. The presence of CD4(+)CD25(+)Foxp3+ Tregs, CD163(+) M2 macrophages and matrix metalloproteinase-9(+) cells was examined immunohistologically in fixed sections of lesional skin from 10 patients with non-invasive extramammary Paget's disease and 7 patients with invasive extramammary Paget's disease. Fewer CD4(+)CD25(+)Foxp3(+) Tregs were observed in non-invasive extramammary Paget's disease than in invasive extramammary Paget's disease. In contrast, higher numbers of CD163(+) macrophages and metalloproteinase-9(+) cells were detected only in invasive extramammary Paget's disease. These findings suggest that the induction of immunosuppressive cells in extramammary Paget's disease differs according to the tumour stage.