Physiological and transcriptomic analyses to determine the responses to phosphorus utilization in Nostoc sp.
Physiological and transcriptomic analyses to determine the responses to phosphorus utilization in Nostoc sp.
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生理和转录组分析以确定发菜对磷利用的反应。
DOI：
10.1016/j.hal.2019.03.002
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发表时间：
2019-04-01
期刊：
影响因子：
6.6
通讯作者：
Wu, Zhongxing
中科院分区：
文献类型：
--
作者：
Dong, Congcong;Zhang, Hongbo;Wu, Zhongxing
磷是水生生态系统中藻类生长的重要驱动因子。本文对发菜的生长、磷的吸收利用、光合作用和转录组进行了研究。在发菜(Nostoc sp.)在含有β-甘油磷酸(β-Gly，含C-O-P键)、2-氨基乙基膦酸(2-Amin，含C-P键)或正磷酸盐(K2HPO4)的培养液中培养，以及在无磷(NP)的培养液中培养。结果表明，NP处理对发菜的生长和光合作用有不利影响。并显著下调与营养物质运输和物质代谢相关的基因表达。此外，2-氨基转移酶抑制了发菜的生长。但没有显著降低光合作用，NP和2-AMIN处理上调了抗氧化剂和胁迫相关基因的表达。此外，β-甘氨酸和K2HPO4处理在生长、光合作用和磷利用方面没有明显差异。这些结果表明，发菜具有灵活的利用磷的能力，这可能是发菜在环境中广泛分布的重要原因。
Phosphorus (P) is an important factor driving algal growth in aquatic ecosystems. In the present study, the growth, P uptake and utilization, photosynthesis, and transcriptome profile of Nostoc sp. were measured when Nostoc sp. cultured in media containing beta-glycerol phosphate (beta-gly, containing C-O-P bonds), 2-aminoethylphosphonic acid (2-amin, containing C-P bonds), or orthophosphate (K2HPO4), and in P-free (NP) medium. The results revealed that NP treatment adversely affected the growth and photosynthesis of Nostoc sp. and significantly down-regulated the expression of genes related to nutrient transport and material metabolism. Furthermore, 2-amin treatment reduced the growth of Nostoc sp. but did not significantly reduce photosynthesis, and the treatments of NP and 2-amin up-regulated the expressions of genes related antioxidation and stress. Additionally, there were no obvious differences in growth, photosynthesis, and phosphorus utilization between the beta-gly and K2HPO4 treatments. These results suggested that Nostoc had a flexible ability to utilize P, which might play an important role in its widespread distribution in the environment.