Hyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: Implications in acute and chronic infections
Hyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: Implications in acute and chronic infections
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DOI：
10.1016/j.archoralbio.2014.06.004
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发表时间：
2014-10-01
影响因子：
3
通讯作者：
Yan, Fuhua
中科院分区：
文献类型：
--
作者：
Chen, Shuai;Lin, Gengbing;Yan, Fuhua
目的：探讨高脂血症对牙周细菌感染免疫应答的影响。方法：雄性新西兰大白兔60只，平均分为正常饲粮（ND）和高脂饲粮（HFD），为期6周。每6只患有ND或HFD的家兔口服活放线菌聚集菌和牙龈卟啉单胞菌，每周3次，连续8周。每6只患ND或HFD的家兔静脉注射放线菌球菌和牙龈卟啉卟啉脂多糖。牙周病的严重程度通过宏观和影像学评估量化。采用酶联免疫吸附法检测血清细胞因子。体外收集外周单核细胞，LPS刺激。采用实时定量聚合酶链反应检测巨噬细胞基因表达的变化。结果：在感染早期，HFD家兔暴露于口腔感染和全身感染，与ND家兔相比，细胞因子表达降低，炎症反应较弱。然而，HFD家兔在长期感染中表现出更高的炎症细胞因子表达。此外，炎症细胞因子表达的显著变化引起口腔感染HFD家兔骨质流失的显著增加。与体外培养的ND兔相比，HFD兔的外周巨噬细胞暴露于LPS后显示出促炎细胞因子水平降低。结论：这些数据表明高脂血症对免疫反应的干扰不同。其机制可能与急性期免疫麻痹和慢性期炎症介质的积累有关。(C) 2014 Elsevier Ltd.版权所有。
Objective: In this study, the effect of hyperlipidemia on immune responses to periodontal bacterial infections was investigated.Methods: Sixty male New Zealand white rabbits were equally assigned to normal diet (ND) and high-fat diet (HFD) for 6 weeks. Every six rabbits with ND or HFD were orally inoculated with live Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis three times a week for 8 weeks. Also every six rabbits with ND or HFD rabbits were injected intravenously with A. actinomycetemcomitans and P. gingivalis LPS. Periodontal disease severity was quantified by macroscopic and radiographical evaluation. Serum cytokines were examined by enzyme-linked immunosorbent assay. In vitro, peripheral mononuclear cells were collected and stimulated with LPS. Quantitative real-time polymerase chain reaction was used to determine the changes in gene expression of macrophages.Results: In the early stages of infection, HFD rabbits were exposed to oral infection and systemic infection developed a weak inflammatory response to the reduced cytokine expression compared with ND rabbits. However, HFD rabbits exhibited higher inflammatory cytokine expression during long-term infections. Moreover, the pronounced changes in inflammatory cytokine expression elicited a significantly increase in bone loss in HFD rabbits with oral infection. Peripheral macrophages harvested from HFD rabbits and exposed to LPS exhibited reduced levels of pro-inflammatory cytokines compared with those from ND rabbits in vitro.Conclusion: These data indicated that hyperlipidemia interfered with immune responses differently. The mechanism is possibly associated with immune paralysis in the acute phase and accumulation of inflammatory mediators in the chronic period. (C) 2014 Elsevier Ltd. All rights reserved.