ROLE OF ADENOVIRUS ANTIGENS IN INDUCTION OF VIRUS NEUTRALIZING ANTIBODY
ROLE OF ADENOVIRUS ANTIGENS IN INDUCTION OF VIRUS NEUTRALIZING ANTIBODY
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DOI：
10.1099/0022-1317-2-1-177
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发表时间：
1968-01-01
影响因子：
3.8
通讯作者：
PEREIRA, HG
中科院分区：
文献类型：
--
作者：
KJELLEN, L;PEREIRA, HG
用纯化的5型腺病毒六邻体、五邻体和纤维抗原制备豚鼠抗血清。通过免疫扩散、补体结合和血凝抑制试验判断与特定抗原特异性反应的血清测定病毒中和能力。后者几乎完全由抗六邻体血清产生。5型抗六邻体而非抗纤维血清中和1型病毒，只要血清病毒反应在低pH值下发生，由于六邻体抗原含有组特异性腺病毒抗原，可通过补体固定或免疫扩散证实，因此测定了异型腺病毒兔抗血清与5型腺病毒联合收割机结合的能力。通过证明加入山羊抗兔血清后的病毒灭活，获得了此类组合的证据。结果表明，1型和2型腺病毒抗血清可与5型病毒结合。所用技术未显示5型病毒与抗3型、12型、21型和抗考克斯-萨奇B的抗血清之间的组合。抗1型、2型和12型兔抗血清的IgG组分显示出增强5型病毒+抗体复合物的灭活。3型或科萨基B、IgG均未增强。结果表明，尽管六邻体抗原含有共同的结构，但不同病毒类型的构型模式有所不同。
Guinea pig antisera were prepared against purified hexon, penton and fibre antigens of adenovirus type 5. Sera reacting specifically with particular antigens as judged by immunodiffusion, complement fixation and haemagglu- tination-inhibition tests were assayed for virus neutralizing capacity. The latter was exerted almost exclusively by anti-hexon sera. Type 5 anti-hexon but not anti-fibre sera neutralized type 1 virus provided the serum virus reaction took place at low pH values.Since the hexon antigens contain the group-specific adenovirus antigen, demonstrable by complement fixation or immunodiffusion, the ability of heterotypic adenovirus rabbit antisera to combine with adenovirus type 5 was assayed. Evidence of such combinations was obtained by the demonstration of virus inactivation following addition of a goat anti-rabbit serum. It was shown that adenovirus type 1 and type 2 antisera combined with type 5 virus. The technique used did not reveal combinations between type 5 virus and antisera against type 3, type 12, type 21, nor against Cox- sackie B. The IgG fractions of rabbit antisera against type 1, type 2 and type 12 were shown to enhance inactivation of type 5 virus+antibody complexes. No enhancement was obtained with type 3 or Coxsackie B, IgG. The results suggest that although the hexon antigens contain common structures the configurational pattern differs among the different virus types.