Tissue engineering of heart valves: PEGylation of decellularized porcine aortic valve as a scaffold for in vitro recellularization.
Tissue engineering of heart valves: PEGylation of decellularized porcine aortic valve as a scaffold for in vitro recellularization.
复制标题
心脏瓣膜的组织工程：脱细胞猪主动脉瓣的聚乙二醇化作为体外再细胞化的支架
DOI：
10.1186/1475-925x-12-87
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发表时间：
2013-09-05
影响因子：
3.9
通讯作者：
Dong N
中科院分区：
文献类型：
--
作者：
Zhou J;Hu S;Ding J;Xu J;Shi J;Dong N
聚乙二醇（PEG）引起了组织工程应用的广泛兴趣。本研究的目的是合成具有二丙烯酸酯末端基团的 4 臂 -PEG-20kDa (4-arm-PEG-DA)，并根据其机械和生物学特性评估其对脱细胞猪主动脉瓣 (DAV) 的双重功能。采用线性PEG 20,000单体接枝共聚合成4臂-PEG-DA，并通过IR1H NMR和13C NMR表征； DAV的聚乙二醇化是通过丙烯酰基与硫醇之间的迈克尔加成反应实现的，通过单轴平面拉伸试验、苏木精和伊红(HE)和扫描电子显微镜(SEM)测试其效果。 Gly-Arg-Gly-Asp-Ser-Pro-Cys (GRGDSPC) 肽和血管内皮生长因子-165 (VEGF165) 通过分支 PEG-DA (GRGDSPC-PEG-DAV-PEG-VEGF165) 缀合到 DAV 上。机械测试证实 PEG 交联显着增强了 DAV 的拉伸强度。免疫荧光证实GRGDSPC肽和VEGF165有效缀合到DAV上；结合的定量大致使用分光光度法和ELISA完成。人脐静脉内皮细胞 (HUVEC) 在 GRGDSPC-PEG-DAV-PEG-VEGF165 上生长和扩散良好。所以，
Poly (ethylene glycol) (PEG) has attracted broad interest for tissue engineering applications. The aim of this study was to synthesize 4-arm -PEG-20kDa with the terminal group of diacrylate (4-arm-PEG-DA) and evaluate its dual functionality for decellularized porcine aortic valve (DAV) based on its mechanical and biological properties. 4-arm-PEG-DA was synthesized by graft copolymerization of linear PEG 20,000 monomers, and characterized by IR1H NMR and 13C NMR; PEGylation of DAV was achieved by the Michael addition reaction between propylene acyl and thiol, its effect was tested by uniaxial planar tensile testing, hematoxylin and eosin (HE) and scanning electron microscopy (SEM). Gly-Arg-Gly-Asp-Ser-Pro-Cys (GRGDSPC) peptides and vascular endothelial growth factor-165 (VEGF165) were conjugated onto DAV by branched PEG-DA (GRGDSPC-PEG-DAV-PEG-VEGF165). Mechanical testing confirmed that PEG-cross-linking significantly enhanced the tensile strength of DAV. Immunofluoresce confirmed the GRGDSPC peptides and VEGF165 were conjugated effectively onto DAV; the quantification of conjunction was completed roughly using spectrophotometry and ELISA. The human umbilical vein endothelial cells (HUVECs) grew and spread well on the GRGDSPC-PEG-DAV-PEG-VEGF165. Therefore,