The Potato ERF Transcription Factor StERF3 Negatively Regulates Resistance to Phytophthora infestans and Salt Tolerance in Potato
The Potato ERF Transcription Factor StERF3 Negatively Regulates Resistance to Phytophthora infestans and Salt Tolerance in Potato
复制标题
马铃薯 ERF 转录因子 StERF3 负调控马铃薯致病疫霉抗性和耐盐性
DOI：
10.1093/pcp/pcv025
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发表时间：
2015-05-01
影响因子：
4.9
通讯作者：
Xie, Conghua
中科院分区：
文献类型：
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作者：
Tian, Zhendong;He, Qin;Xie, Conghua
乙烯反应因子(ERF)是植物界所特有的，在植物对各种生物和非生物胁迫的响应中起着至关重要的作用。我们在这里展示了一个马铃薯StERF3，它在其C-末端含有ERF相关的两亲性抑制(EAR)基序，负调控马铃薯对晚疫病菌的抗性和耐盐性。StERF3启动子对水杨酸、ABA乙烯和氯化钠以及马铃薯晚疫病病原菌致病疫霉的诱导有反应。在酵母细胞中，StERF3能与His3启动子的GCC盒元件结合，激活His3的转录。重要的是，在马铃薯中沉默StERF3提高了叶片对致病疫霉的抗性，并提高了植物对盐胁迫的耐受性，同时伴随着防御相关基因(PR1、NPR1和WRKY1)的激活。相反，过度表达StERF3的植株表现出这些防御相关基因的表达减少，并增强了对致病疫霉的敏感性，这表明StERF3在马铃薯下游的防御和/或胁迫相关基因中起着负调控作用。StERF3定位于细胞核。有趣的是，酵母双杂交试验和双分子荧光互补(BIFC)试验表明，StERF3可以与细胞质中的其他蛋白质相互作用，这可能导致其在细胞核和细胞质之间的重新定位，揭示了一种新的StERF3调控手段。综上所述，这些数据为揭示StERF3负性调控马铃薯晚疫病抗性和非生物耐性的机制提供了新的见解，并可能在马铃薯晚疫病抗性工程中有潜在的应用。
Ethylene response factors (ERFs) are unique to the plant kingdom and play crucial roles in plant response to various biotic and abiotic stresses. We show here that a potato StERF3, which contains an ERF-associated amphiphilic repression (EAR) motif in its C-terminal region, negatively regulates resistance to Phytophthora infestans and salt tolerance in potato. The StERF3 promoter responds to induction by salicylic acid, ABA ethylene and NaCl, as well as P. infestans, the causal agent of potato late blight disease. StERF3 could bind to the GCC box element of the HIS3 promoter and activate transcription of HIS3 in yeast cells. Importantly, silencing of StERF3 in potato produced an enhanced foliage resistance to P. infestans and elevated plant tolerance to NaCl stress accompanied by the activation of defense-related genes (PR1, NPR1 and WRKY1). In contrast, StERF3-overexpressing plants showed reduced expression of these defense-related genes and enhanced susceptibility to P. infestans, suggesting that StERF3 functions as a negative regulator of downstream defense- and/or stress-related genes in potato. StERF3 is localized to the nucleus. Interestingly, yeast two-hybrid assay and a bimolecular fluorescence complementation (BiFC) test clarified that StERF3 could interact with other proteins in the cytoplasm which may lead to its re-localization between the nucleus and cytoplasm, revealing a novel means of StERF3 regulation. Taken together, these data provide new insights into the mechanism underlying how StERF3 negatively regulates late blight resistance and abiotic tolerance in potato and may have a potential use in engineering late blight resistance in potato.