Estrogenic activity and reproductive effects of the UV-filter oxybenzone (2-hydroxy-4-methoxyphenyl-methanone) in fish
Estrogenic activity and reproductive effects of the UV-filter oxybenzone (2-hydroxy-4-methoxyphenyl-methanone) in fish
复制标题
DOI：
10.1016/j.aquatox.2008.08.018
复制
发表时间：
2008-11-21
影响因子：
4.5
通讯作者：
Schlenk, Daniel
中科院分区：
文献类型：
--
作者：
Coronado, Michael;De Haro, Hector;Schlenk, Daniel
先前对美国加利福尼亚州海岸市政排水口周围沉积物和美国纽约州纽约市污水提取物的研究表明，紫外线过滤剂氧苯酮（CAS# 131-57-7；二苯甲酮-3）是一种潜在的雌激素。使用为期 14 天的幼年虹鳟鱼血浆卵黄蛋白原测定和随后的 21 天日本青鳉繁殖测定来评估氧苯酮对雌激素活性和繁殖的影响。在1000μg/L的氧苯酮标称浓度（749μg/L中值测量值）下，在虹鳟鱼中观察到卵黄蛋白原的显着诱导，该浓度比之前废水排放中观察到的浓度高约75倍。在雄性日本青鳉 (Oryzias latipes) 暴露 21 天后，在标称浓度为 1000 μ g/L（测量中位数为 620 μ g/L）的氧苯酮中也观察到卵黄蛋白原诱导。暴露于相同浓度（620μg/L）的每只雌性每天产卵数在7天后显着降低，但在21天后恢复到对照值。然后对受精卵进行为期 20 天的监测，以评估孵化成功率。在 620 μg/L 氧苯酮浓度下，21 天暴露期间收集到的受精卵孵化的总体百分比明显较低。该浓度还存在时间效应，因为收集蛋后 13-15 天，蛋的活力（孵化的受精蛋的百分比）降低。所有三种羟苯甲酮浓度（16、132 和 620 μg/L）和 50 ng/L 雌二醇阳性对照均显示第 15 天的卵孵化减少，并且 132 和 620 μg/L 羟苯甲酮浓度降低了第 13-15 天孵化的卵的百分比。这些数据表明，紫外线过滤剂氧苯酮会改变两种鱼类的内分泌或繁殖终点，但浓度显着高于环境中测量的浓度。 (C) 2008 Elsevier B.V. 保留所有权利。
Previous studies in extracts of sediments surrounding municipal outfalls off the coast of California, USA and effluents of New York City, NY, USA indicated the UV-filtering agent, oxybenzone (CAS# 131-57-7; benzophenone-3) as a potential estrogen. The effects of oxybenzone on estrogenic activity and reproduction were evaluated using a 14-day juvenile rainbow trout assay for plasma vitellogenin and a subsequent 21-day Japanese medaka reproduction assay. Significant induction of vitellogenin was observed in the rainbow trout at the 1000 mu g/L nominal concentration (749 mu g/L median measured value) of oxybenzone which was approximately 75 times greater than the concentrations observed in previous wastewater effluent. Vitellogenin induction was also observed in the 1000 mu g/L nominal concentration (620 mu g/L median measured) of oxybenzone in male Japanese medaka (Oryzias latipes) after 21 days of exposure. The number of eggs produced per female per day exposed to the same concentration (620 mu g/L) were significantly lower after 7 days, but returned to control values after 21 days. Fertilized eggs were then monitored for 20 days to assess hatching success. The overall percentage of fertilized eggs collected during the 21-day exposure that hatched was significantly lower in the 620 mu g/L oxybenzone concentration. There was also a temporal effect at this concentration as egg viability (percentage of fertilized eggs that hatched) was diminished 13-15 days after eggs were collected. All three oxybenzone concentrations (16, 132, and 620 mu g/L) and the 50 ng/L estradiol positive control showed reduced hatching of eggs at day 15, and the 132 and 620 mu g/L oxybenzone concentrations diminished the percentage of eggs that hatched on days 13-15. These data indicate that the UV-filter oxybenzone alters endocrine or reproduction endpoints in two fish species, but at concentrations significantly higher than those measured in the environment. (C) 2008 Elsevier B.V. All rights reserved.