Correlation Between Tumor Necrosis Factor-α and Interleukin-1β in Exhaled Breath Condensate and Pulmonary Function
Correlation Between Tumor Necrosis Factor-α and Interleukin-1β in Exhaled Breath Condensate and Pulmonary Function
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DOI：
10.1016/j.amjms.2017.06.004
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发表时间：
2017-10-01
影响因子：
3.1
通讯作者：
Sun, Daqiang
中科院分区：
文献类型：
--
作者：
Lin, Xuefeng;Fan, Yichu;Sun, Daqiang
背景：呼出气冷凝液（EBC）已成为评估肺部疾病炎症的一种非侵入性方法。我们的目的是研究肿瘤坏死因子α与肿瘤坏死因子受体之间的相关性。（INF-α）和白细胞介素1 β方法：将60例早期肺癌切除患者随机分为3组，每组10例，分别测定EBC和肺组织中IL-1 β的含量，并与COPD患者的肺功能进行比较。COPD治疗组、COPD对照组和非COPD组。COPD治疗组的患者除了胸部物理治疗外，还接受了所谓的“肺保护治疗”，包括氨溴索、布地奈德和异丙托溴铵。患者接受肺功能检测和EBC收集，并通过酶联免疫吸附试验（ELISA）检测TNF-α和IL-1 β。采用免疫荧光法检测肺组织中TNF-α和IL-1 β。结果：COPD组EBC中TNF-α和IL-1 β水平术前均显著高于非COPD组（均P < 0.01），术前给予肺保护治疗后，TNF-α和IL-1 β水平均显著降低（均P <0.01）。所有患者在手术后接受肺保护治疗后，EBC中TNF-α和IL-1 β水平均显著降低（P = 0.027，P = 0.004）。COPD组肺组织中TNF-α和IL-1 β含量显著高于对照组（均P < 0.05），组织学分析结果相似。FEV 1/FVC与TNF-α和IL-1 β表达呈负相关。结论：EBC中TNF-α和IL-1 β是评价COPD患者肺功能和炎症反应的潜在生物标志物。此外，肺保护性治疗可有效减轻COPD患者的炎症。
Background: Exhaled breath condensate (EBC) has emerged as a noninvasive method for assessing inflammation in lung diseases. Our aim is to investigate the correlation between tumor necrosis factor alpha (INF-alpha) and interleukin 1 beta (IL-1 beta) in EBC and in lung tissue, and between these values in EBC with pulmonary function tests in patients with chronic obstructive pulmonary disease (COPD).Materials and Methods: To ensure the availability of lung tissue, 60 patients undergoing resection for early lung cancer were divided into 3 groups: a COPD treatment group, a COPD control group and a non-COPD group. Patients in the COPD treatment group received what was termed "lung-protective treatment" including ambroxol, budesonide and ipratropium bromide in addition to chest physiotherapy. Patients underwent pulmonary function testing and EBC collection, and TNF-alpha and IL-1 beta were detected by enzyme-linked immunosorbent assay (ELISA). TNF-alpha and IL-1 beta in lung tissues were evaluated by immunoflorescense. Correlations were analyzed by Pearson correlation coefficients.Results: The TNF-alpha and IL-1 beta levels in EBC were significantly higher in the COPD groups compared with the non-COPD group before surgery (all P < 0.01), and the levels were significantly decreased after lung-protective treatment was received before surgery (all P < 0.01). TNF-alpha and IL-1 beta levels in EBC were significantly decreased in all patients after surgery with lung-protective treatment (P = 0.027, P = 0.004). TNF-alpha and IL-1 beta content in lung tissues was significantly higher in the COPD groups (all P < 0.05), and the histologic analysis showed similar results. Negative correlations between FEV1/FVC and expression of TNF-alpha and IL-1 beta were observed. There was a positive correlation between TNF-alpha and IL-1 beta in lung tissues and in EBC.Conclusions: TNF-alpha and IL-1 beta in EBC are potential biomarkers for evaluating pulmonary function and inflammation in patients with COPD. Furthermore, lung-protective treatment is effective in reducing inflammation in patients with COPD.