Sensitive low-light-level microspectrophotometer: detection of photosensitive pigments of retinal cones.
Sensitive low-light-level microspectrophotometer: detection of photosensitive pigments of retinal cones.
复制标题
灵敏微光分光光度计：检测视网膜锥体的光敏色素。
DOI：
10.1364/josa.54.001451
复制
发表时间：
1964
影响因子：
--
通讯作者：
G. Entine
中科院分区：
文献类型：
--
作者：
P. Liebman;G. Entine
本文报道了一种高灵敏度的显微分光光度计，它能在最低光下记录单个细胞视色素的吸收光谱。使用该设备，在小于1 μ半径的区域中记录了单个视锥细胞的色素，并且漂白最小。该仪器设计简单灵活，并始终使用市售组件。本文回顾了一些一般的设计考虑，我们的设备的特点和性能，以及一些视网膜锥获得的结果。
A microspectrophotometer of exceptional sensitivity has been constructed to record absorption spectra of visual pigments in single cells using minimum light. With this equipment, pigments of individual cones have been recorded in regions of less than 1 μ radius, with minimum bleaching. The instrument is simple and flexible in design, and uses commercially available components throughout. This paper reviews some general design considerations, the character and performance of our equipment, and some of the results obtained with retinal cones.