Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts
Effects of human neutrophil peptide-1 on the expression of interstitial collagenase and type I collagen in human dermal fibroblasts
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DOI：
10.1007/s00403-002-0310-6
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发表时间：
2002-07-01
影响因子：
3
通讯作者：
Iwatsuki, K
中科院分区：
文献类型：
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作者：
Oono, T;Shirafuji, Y;Iwatsuki, K
人中性粒细胞肽-1（HNP-1）是一种具有抗菌特性的防御素，也被认为可以促进伤口愈合。为了阐明该因子促进伤口愈合的机制，我们通过RT-PCR和ELISA方法研究了HNP-1对培养的成纤维细胞表达间质胶原酶（基质金属蛋白酶1，MMP-1）、I型和III型胶原以及金属蛋白酶组织抑制剂1（TIMP-1）的影响。我们的研究结果表明，合成HNP-1增加前α I（I）胶原mRNA和蛋白的表达。与此相反，MMP-1的表达在mRNA和蛋白水平上均降低。我们的观察结果表明，HNP-1可能通过增强细胞外基质沉积和控制其降解来促进伤口修复。
Human neutrophil peptide-1 (HNP-1), a defensin with antimicrobial properties, is also thought to promote wound healing. To elucidate the mechanism by which wound healing is facilitated by this factor, we investigated the effect of HNP-1 on the expression of interstitial collagenase (matrix metalloproteinase 1, MMP-1), collagen types I and III, and tissue inhibitor of metalloproteinase 1 (TIMP-1) by cultured fibroblasts by means of RT-PCR and ELISA. Our results showed that synthetic HNP-1 increased the expression of proalphaI(I) collagen mRNA and protein. In contrast, the expression of MMP-1 was decreased at both the mRNA and protein levels. Our observations suggest that HNP-1 may promote wound repair by enhancing extracellular matrix deposition and by controlling its degradation.