A modified choline-deficient, ethionine-supplemented diet protocol effectively induces oval cells in mouse liver
A modified choline-deficient, ethionine-supplemented diet protocol effectively induces oval cells in mouse liver
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DOI：
10.1053/jhep.2001.26751
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发表时间：
2001-09-01
期刊：
影响因子：
13.5
通讯作者：
Yeoh, GC
中科院分区：
文献类型：
--
作者：
Akhurst, B;Croager, EJ;Yeoh, GC
有几种可靠且可重复的方法可用于诱导大鼠肝脏中的卵圆细胞。有效的方法通常包括使用烷化剂抑制肝细胞增殖，然后对大鼠进行部分肝切除（PH）。这种手术在小鼠中难以重复进行。不包括部分肝切除的方法，例如给予D - 半乳糖胺，在小鼠中是无效的。我们发现，胆碱缺乏、补充乙硫氨酸（CDE）的饮食在大鼠中非常有效，但给予小鼠时会导致高发病率和死亡率。本文概述了一种可用于给小鼠喂食CDE饮食的替代方案。这种饮食被证明对诱导卵圆细胞非常有效，且不会导致高死亡率。它耗时更短，并且至少与其他常用的诱导小鼠卵圆细胞的方案一样有效。
Several reliable and reproducible methods are available to induce oval cells in rat liver. Effective methods often involve inhibiting proliferation in hepatocytes using an alkylating agent, then subjecting the rat to partial hepatectomy (PH). The surgery is difficult to perform reproducibly in mice. Approaches that do not include partial hepatectomy, such as administration of D-galactosamine, are ineffective in mice. We found that a choline-deficient, ethionine-supplemented (CDE) diet, which is very effective in rats, leads to high morbidity and mortality when administered to mice. This article outlines an alternative protocol by which a CDE diet can be administered to mice. This diet is shown to be highly effective for oval cell induction, without causing high mortality. It takes less time and is at least as effective as other commonly used protocols for inducing oval cells in mice.