Two subtypes (subgenotypes) of hepatitis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism
Two subtypes (subgenotypes) of hepatitis B virus genotype C: A novel subtyping assay based on restriction fragment length polymorphism
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DOI：
10.1016/j.hepres.2005.08.006
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发表时间：
2005-11-01
影响因子：
4.2
通讯作者：
Mizokami, M
中科院分区：
文献类型：
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作者：
Tanaka, Y;Orito, E;Mizokami, M
近期，乙型肝炎病毒C基因型（HBV/C）在地理上被典型地分为两个亚型（亚基因型）：东南亚的HBV/C1（Cs）和东亚的HBV/C2（Cc）。我们的目的是开发一种快速的亚型分析方法，并研究这两个亚型的病毒学特征。基于从数据库中获取的171株HBV/C病毒株，在两个亚型之间发现了17个单核苷酸多态性（SNP）。利用非重叠聚合酶区域的5个SNP，新开发了一种使用三种内切核酸酶的限制性片段长度多态性方法，用于区分HBV/Cs和HBV/Cc。该方法应用于来自日本和中国香港的49名HBV/C携带者。中国香港的24例被归类为HBV/Cs，日本的25例为HBV/Cc，经测序证实。在衣壳化信号中检测到一些特定的突变；在HBV/Cc病毒株中发现前C区终止突变（A1896），同时在1858位核苷酸处有C→T替换，而在HBV/Cs中发现另一种前C区突变（A1898），同时在1856位核苷酸处有C→T突变。特别是，HBV/Cc中的两个紧密连锁的突变（A1896和A1899）能够更有效地稳定ε环结构并影响病毒复制。因此，这两个亚型之间的这些病毒学差异可能会影响临床特征。（c）2005爱思唯尔爱尔兰有限公司。保留所有权利。
Recently hepatitis B virus genotype C (HBV/C) has been classified into geographically typical two subtypes (subgenotypes); HBV/C I in Southeast Asia (Cs) and HBV/C2 in East Asia (Cc). Our aim is to develop a rapid subtyping assay and to examine the virological features of these two subtypes. Based on 171 HBV/C strains retrieved from the database, 17 single nucleotides polymorphisms (SNPs) were found between two subtypes. Taking advantage of five SNPs in non-overlapping polymerase region, a restriction fragment length polymporphism ,method with three endonucleases was newly developed for distinguishing between HBV/Cs and HBV/Ce. The method was applied to 49 HBV/C carriers from Japan and Hong Kong. The 24 in Hong Kong were classified into HBV/Cs, and the 25 in Japan were HBV/Ce, confirmed by sequencing. Some specific mutations were detected in the encapsidation signal; precore stop mutation (A 1896), accompanied by a C-to-T substitution at nt 1858, was found in HBV/Ce strains, and another precore mutation (A] 898), accompanied by a C-to-T mutation at nt 1856, was found in HBV/Cs. Especially, two closely linked mutations (A 1896 and A 1899) in HBV/Ce could stabilize the epsilon loop structure more efficiently and influece viral replication. Hence, these virological differences between the two subtypes might influence clinical features. (c) 2005 Elsevier Ireland Ltd. All rights reserved.