Symmetrical mutant phenotypes of the receptor EphB4 and its specific transmembrane ligand ephrin-B2 in cardiovascular development
Symmetrical mutant phenotypes of the receptor EphB4 and its specific transmembrane ligand ephrin-B2 in cardiovascular development
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DOI：
10.1016/s1097-2765(00)80342-1
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发表时间：
1999-09-01
期刊：
影响因子：
16
通讯作者：
Anderson, DJ
中科院分区：
文献类型：
--
作者：
Gerety, SS;Wang, HU;Anderson, DJ
Ephrin-B2是一种跨膜配体，在动脉而非静脉中特异性表达，对心血管发育至关重要。然而，ephrin-B2也在非血管组织中表达，并与内皮细胞和非内皮细胞中表达的多种EphB类受体相互作用。因此，ephrin-B2相关受体的身份和这些分子相互作用以控制血管生成的位点尚不清楚。我们发现EphB4是一种ephrin-B2的特异性受体，它只在胚胎血管内皮细胞中表达，并优先在静脉中表达。EphB4的靶向突变本质上复制了ephrin-B2的突变。这些数据表明，ephrin-B2-EphB4相互作用在血管内皮细胞中是必需的，并且与它们介导血管生成所必需的双向信号的观点是一致的。
Ephrin-B2 is a transmembrane ligand that is specifically expressed on arteries but not veins and that is essential for cardiovascular development. However, ephrin-B2 is also expressed in nonvascular tissues and interacts with multiple EphB class receptors expressed in both endothelial and nonendothelial cell types. Thus, the identity of the relevant receptor for ephrin-B2 and the site(s) where these molecules interact to control angiogenesis were not clear. Here we show that EphB4, a specific receptor for ephrin-B2, is exclusively expressed by vascular endothelial cells in embryos and is preferentially expressed on veins. A targeted mutation in EphB4 essentially phenocopies the mutation in ephrin-B2. These data indicate that ephrin-B2-EphB4 interactions are intrinsically required in vascular endothelial cells and are consistent with the idea that they mediate bidirectional signaling essential for angiogenesis.