Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage.
Permanent lymphoid lines from genetically marked lymphocytes: success with lymphocytes recovered from frozen storage.
复制标题
来自基因标记淋巴细胞的永久淋巴系：成功地从冷冻储存中恢复了淋巴细胞。
作者：
William S. Sly;Gurbax S. Sekhon;Roger Kennett;Walter F. Bodmer;J. G. Bodmer
外周血淋巴细胞经分离HL-A分型并在液氮中保存两年，成功建立了永久人淋巴细胞系。冷冻淋巴细胞选自在 LA 和四个 HL-A 基因座纯合的两个兄弟姐妹。用狨猴淋巴系 B95-8 产生的 EB 病毒转化解冻的淋巴细胞。使用 G 和 Q 显带技术对来自已建立的人淋巴系的细胞制备的核型没有发现染色体异常。 HL-A 分型显示了预期的 HL-A 抗原以及大量其他反应。分离淋巴细胞并将其冷冻以供将来使用需要相对较小的投资。这种保存细胞的方法可以应用于患有有趣的遗传疾病或其他生化标记的患者，以提供可以在数年后转化和繁殖的细胞。
Permanent human lymphoid cell lines were established successfully from peripheral blood lymphocytes which had been separated for HL-A typing and stored in liquid nitrogen for two years. Frozen lymphocytes were chosen from two siblings who were homozygous at the LA and FOUR HL-A loci. Thawed lymphocytes were transformed with EB virus produced by the marmoset lymphoid line B95-8. No chromosome abnormalities were seen on karyotypes prepared on cells from the established human lymphoid lines using G and Q banding techniques. HL-A typing showed the expected HL-A antigens plus a considerable number of additional reactions. Separation of lymphocytes and freezing them for possible future use requires a relatively small investment. This method of preserving cells can be applied to patients with interesting genetic disorders or other biochemical markers to provide cells which can be transformed and propagated years later.