Xenopus Cdc6 confers sperm binding competence to oocytes without inducing their maturation.
Xenopus Cdc6 confers sperm binding competence to oocytes without inducing their maturation.
复制标题
爪蟾 Cdc6 赋予卵母细胞精子结合能力，而不诱导其成熟。
DOI：
10.1073/pnas.94.20.10729
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发表时间：
1997
影响因子：
11.1
通讯作者：
Lennarz,WJ
中科院分区：
文献类型：
--
作者：
Tian,J;Thomsen,GH;Gong,H;Lennarz,WJ
两栖类卵通常需要减数分裂成熟才能受精。这一事件的必要前提是精子结合，我们证明在正常的生理条件下，这一特性是在减数分裂成熟时获得的，而不是在减数分裂成熟之前获得的。未成熟卵母细胞不结合精子，但在未成熟卵母细胞中注射总卵子Poly(A)+mRNA可以在没有减数分裂成熟的情况下实现精子结合。通过表达克隆的方法，我们从卵子中分离到了一个可以诱导未成熟卵母细胞结合精子的基因。该基因被发现编码XenopusCDC6，这是一种先前已被证明具有启动DNA复制和细胞周期控制功能的蛋白质。这一出人意料的发现提供了细胞周期调节与影响配子结合的细胞表面属性变化之间的联系的证据。
Amphibian eggs normally require meiotic maturation to be competent for fertilization. A necessary prerequisite for this event is sperm binding, and we show that under normal physiological conditions this property is acquired at, but not before, meiotic maturation. Immature oocytes do not bind sperm, but injection of total egg poly(A)+mRNA into immature oocytes confers sperm binding in the absence of meiotic maturation. Using an expression cloning approach we have isolated a single cDNA from egg poly(A)+mRNA that can induce sperm binding in immature oocytes. The cDNA was found to encodeXenopusCdc6, a protein that previously has been shown to function in initiation of DNA replication and cell cycle control. This unanticipated finding provides evidence of a link between a regulator of the cell cycle and alterations in cell surface properties that affect gamete binding.