Effects of cello-oligosaccharide on intestinal microbiota and epithelial barrier function of weanling pigs
Effects of cello-oligosaccharide on intestinal microbiota and epithelial barrier function of weanling pigs
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纤维寡糖对断奶仔猪肠道菌群及上皮屏障功能的影响
DOI：
10.2527/jas.2014-8248
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发表时间：
2015-03-01
影响因子：
3.3
通讯作者：
Shi, B.
中科院分区：
文献类型：
--
作者：
Jiao, L. F.;Ke, Y. L.;Shi, B.
试验选用144头21 +/- 1日龄断奶仔猪（杜洛克×长白×约克郡，平均初重6.13 kg），分为4个处理，每组6个栏，每个栏6头猪，为期2周。饲喂试验结束后，每组屠宰6头猪，研究饲粮中添加纤维素寡糖（COS）对仔猪肠道菌群和上皮屏障功能的影响。以玉米为代价，在基础饲粮中分别添加0、1.5、3.0和4.5 g/kg COS。采用血浆d -乳酸、二胺氧化酶（DAO）和Ussing chamber技术测定肠屏障功能。采用基于16S rrna的方法进行肠道菌群分析。结果表明，添加COS对生长性能无显著影响（P < 0.05）。增加COS水平可使空肠和结肠内容物中乳酸菌增多（P < 0.05）；空肠内容物中猪链球菌含量降低（P < 0.05），结肠内容物中猪链球菌和大肠杆菌含量降低（P < 0.05）；血浆DAO降低（线性，P = 0.013，二次，P = 0.037）；空肠黏膜DAO增加（线性，P = 0.003，二次，P = 0.008）；空肠和结肠异硫氰酸葡聚糖荧光素4 kDa通量降低（P < 0.05）；结肠经皮电阻（TER）升高（P < 0.05），空肠黏膜claudin-1蛋白表达升高（线性，P = 0.001，二次型，P = 0.003），结肠黏膜claudin-1和zzo -1蛋白表达呈线性（P = 0.001, P = 0.001）和二次型（P = 0.001, P = 0.002）。结果表明，COS改善了肠道微生物生态系统，可能有助于改善肠道屏障功能和紧密连接蛋白。结果还表明，在本试验条件下，断奶仔猪饲粮中COS的适宜添加水平为3.0 g/kg。
A total of 144 piglets (Duroc x Landrace x Yorkshire; average initial weight of 6.13 kg weaned at 21 +/- 1 d age) were allotted to 4 treatments for 2 wk, each of which had 6 pens with 6 pigs per pen. After the feeding experiment, 6 pigs per treatment were slaughtered to investigate the effects of cello-oligosaccharide (COS) on intestinal microbiota and epithelial barrier function. The COS was added to the basal diet at 0, 1.5, 3.0, and 4.5 g/kg diet at the expense of corn, respectively. Plasma D-lactate, diamine oxidase (DAO), and the Ussing chamber technique were used to determine the intestinal barrier function. 16S rRNA-based methods were used for intestinal microbiota analysis. The results showed that incremental levels of COS had no effect (P > 0.05) on growth performance. Incremental levels of COS increased lactobacilli in jejunal and colonic contents (P < 0.05); decreased Streptococcus suis in jejunal contents (P < 0.05) and S. suis and Escherichia coli in colonic contents (P < 0.05); reduced plasma DAO (linear, P = 0.013, and quadratic, P = 0.037); increased jejunal mucosa DAO (linear, P = 0.003, and quadratic, P = 0.008); decreased fluorescein isothiocyanate dextran 4 kDa flux of jejunum and colon (P < 0.05); and increased transepithelial electrical resistance (TER) in colon (P < 0.05), claudin-1 protein expression in jejunal mucosa (linear, P = 0.001, and quadratic, P = 0.003), and protein expressions of claudin-1 and zonula occludens-1 (ZO-1) in colonic mucosa linearly (P = 0.001 and P = 0.001, respectively) and quadratically (P = 0.001 and P = 0.002, respectively). The results indicated that the improved microbial ecosystem in the presence of COS might contribute to improvement in intestinal barrier function and tight junction proteins. Results also showed that the appropriate dietary COS supplementation level was 3.0 g/kg in weaned pig diets under our trial conditions.