Generation and Characterization of Mouse Models for Skeletal Disease.
Generation and Characterization of Mouse Models for Skeletal Disease.
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DOI：
10.1007/978-1-0716-0989-7_11
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Williams BO
中科院分区：
文献类型：
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作者：
Foxa GE;Liu Y;Turner LM;Robling AG;Yang T;Williams BO
我们的实验室已经使用基因工程小鼠模型（GEMM）来评估遗传因素对骨质疏松症和骨关节炎等骨骼疾病的影响。对OA的遗传贡献的研究通常通过评估GEMM如何对诱导OA症状模型的手术方法做出反应来完成。在这里，我们将描述的协议，概述了诱导实验OA小鼠以及使用微计算机断层扫描和骨骼组织形态学分析骨骼表型的方法的详细描述。
Our laboratories have used genetically engineered mouse models (GEMMs) to assess genetic contributions to skeletal diseases such as osteoporosis and osteoarthritis. Studies on the genetic contributions to OA are often done by assessing how GEMMs respond to surgical methods that induce symptoms modeling OA. Here, we will describe protocols outlining the induction of experimental OA in mice as well as detailed descriptions of methods for analyzing skeletal phenotypes using micro-computerized tomography and skeletal histomorphometry.