Proteomic analysis profile of engineered articular cartilage with chondrogenic differentiated adipose tissue-derived stem cells loaded polyglycolic acid mesh for weight-bearing area defect repair.
Proteomic analysis profile of engineered articular cartilage with chondrogenic differentiated adipose tissue-derived stem cells loaded polyglycolic acid mesh for weight-bearing area defect repair.
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DOI：
10.1089/ten.tea.2013.0205
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发表时间：
2013-11
影响因子：
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通讯作者：
Lunli Gong;Xiao Zhou;Yaohao Wu;Yun Zhang;Chen Wang;Heng Zhou;Fangfang Guo;Lei Cui
中科院分区：
文献类型：
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作者：
Lunli Gong;Xiao Zhou;Yaohao Wu;Yun Zhang;Chen Wang;Heng Zhou;Fangfang Guo;Lei Cui
本研究旨在探讨利用软骨分化的自体脂肪源性干细胞（ASCs）修复猪关节软骨（AC）负重区全层缺损的可能性，随访3个月和6个月，延续我们之前对非负重区缺损的研究。将分离的ASCs体外植入磷酸甘油/聚乳酸（PGA/PLA）诱导成软骨2周，作为实验组，然后植入猪AC缺损（直径8 mm，深度至软骨下骨），PGA/PLA仅作为对照组。随访3个月和6个月，实验组新生软骨均与邻近正常软骨和软骨下骨在组织学上融合良好，而对照组仅与纤维组织融合。免疫组织化学和甲苯胺蓝染色证实再生软骨中COL II和糖胺聚糖的分布与天然软骨相似。随着修复时间的延长，新生软骨也经历了生动的重塑过程，压缩模量从3个月时正常软骨的70%显著增加到6个月时的近90%，这与我们之前的研究相似。尽管如此，再生软骨仍然可以发现与原生软骨的差异。同时，PGA/PLA介导的ASCs向软骨细胞分化的确切机制尚不清楚。因此，利用蛋白质组学，从20个选择的二维点上鉴定出43种蛋白质，这确实有助于我们进一步研究一些承诺的因素。综上所述，通过蛋白质组学的比较，对ASC向软骨细胞分化的机制有了更深入的了解，本研究进一步证实了这一点，是对非负重地区ASC向软骨细胞分化的完美补充。
The present study was designed to investigate the possibility of full-thickness defects repair in porcine articular cartilage (AC) weight-bearing area using chondrogenic differentiated autologous adipose-derived stem cells (ASCs) with a follow-up of 3 and 6 months, which is successive to our previous study on nonweight-bearing area. The isolated ASCs were seeded onto the phosphoglycerate/polylactic acid (PGA/PLA) with chondrogenic induction in vitro for 2 weeks as the experimental group prior to implantation in porcine AC defects (8 mm in diameter, deep to subchondral bone), with PGA/PLA only as control. With follow-up time being 3 and 6 months, both neo-cartilages of postimplantation integrated well with the neighboring normal cartilage and subchondral bone histologically in experimental group, whereas only fibrous tissue in control group. Immunohistochemical and toluidine blue staining confirmed similar distribution of COL II and glycosaminoglycan in the regenerated cartilage to the native one. A vivid remolding process with repair time was also witnessed in the neo-cartilage as the compressive modulus significantly increased from 70% of the normal cartilage at 3 months to nearly 90% at 6 months, which is similar to our former research. Nevertheless, differences of the regenerated cartilages still could be detected from the native one. Meanwhile, the exact mechanism involved in chondrogenic differentiation from ASCs seeded on PGA/PLA is still unknown. Therefore, proteome is resorted leading to 43 proteins differentially identified from 20 chosen two-dimensional spots, which do help us further our research on some committed factors. In conclusion, the comparison via proteome provided a thorough understanding of mechanisms implicating ASC differentiation toward chondrocytes, which is further substantiated by the present study as a perfect supplement to the former one in nonweight-bearing area.