Indole-3-butyric acid in Arabidopsis thaliana
Indole-3-butyric acid in Arabidopsis thaliana
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拟南芥中的吲哚-3-丁酸
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
E. Epstein
中科院分区：
文献类型：
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作者：
J. Ludwig;E. Epstein
拟南芥（Arabidopsis thaliana）在无菌条件下培养的液体培养基中代谢吲哚-3-丁酸（IBA）为两种主要代谢物。其中一种代谢物经1 N NaOH和β-葡萄糖苷酶水解，初步鉴定为iba -葡萄糖；另一种代谢物经7 N NaOH和氨基酶水解，初步鉴定为酰胺连接的缀合物。在3H-IBA、IBA和udp -葡萄糖孵育后，在可溶性酶组分中发现IBA-葡萄糖合成酶活性。标记的反应产物具有与IBA葡萄糖相当的Rf值，并用Ehmann试剂染色呈阳性。
Indole-3-butyric acid (IBA) was metabolized by seedlings of Arabidopsis thaliana cultivated in liquid medium under sterile conditions to two major metabolites. One metabolite was hydrolyzed by 1 N NaOH and β-glucosidase and was tentatively identified as IBA-glucose and the other was hydrolyzed by 7 N NaOH and amidase and was identified as an amide-linked conjugate. IBA-glucose synthase activity was found in a soluble enzyme fraction after incubation of 3H-IBA, IBA and UDP-glucose. The labelled reaction product had an Rf value comparable to IBA glucose and stained positive with Ehmann reagent.