ENHANCED CONJUGATIVE TRANSFER OF PLASMID DNA FROM ESCHERICHIA-COLI TO STAPHYLOCOCCUS-AUREUS AND LISTERIA-MONOCYTOGENES
ENHANCED CONJUGATIVE TRANSFER OF PLASMID DNA FROM ESCHERICHIA-COLI TO STAPHYLOCOCCUS-AUREUS AND LISTERIA-MONOCYTOGENES
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DOI：
10.1016/0378-1097(93)90436-6
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发表时间：
1993-05-01
影响因子：
2.1
通讯作者：
COURVALIN, P
中科院分区：
文献类型：
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作者：
TRIEUCUOT, P;DERLOT, E;COURVALIN, P
通过接合将可移动穿梭克隆载体从大肠杆菌转移至金黄色葡萄球菌的频率非常低（交配期后每个供体集落形成单位10（-9）个接合转移子）。结果表明，在交配培养基中加入亚抑菌浓度的青霉素（苯唑西林或青霉素G），通过接合来自大肠杆菌的穿梭载体pAT 18，可增加转移频率。coli SM 10转化S.金黄色葡萄球菌80 CR 5 Str（54倍）和单核细胞增生李斯特菌L017 RF（45倍）。这些结果表明，革兰氏阳性菌的细胞壁构成了接合转移E.杆菌还证明了S.金黄色葡萄球菌受体是引入外源DNA的主要障碍。
Transfer of mobilizable shuttle cloning vectors by conjugation from Escherichia coli to Staphylococcus aureus occurred at a very low frequency (10(-9) transconjugants per donor colony-forming unit after the mating period). It was observed that subinhibitory concentrations of penicillins (oxacillin or penicillin G) in the mating medium resulted in increased transfer frequency by conjugation of the shuttle vector pAT18 from E. coli SM10 to S. aureus 80CR5 Str (54-fold) and to Listeria monocytogenes L017RF (45-fold). These results were interpreted as indicating that the cell wall of Gram-positive bacteria constitutes an important barrier for conjugative transfer of genetic information delivered from E. coli. It was also demonstrated that presence of a restriction system(s) in S. aureus recipients represented a major barrier to introduction of foreign DNA.