Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22.
Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22.
复制标题
芽殖酵母起源识别复合体亚基 ORC5 的人类和裂殖酵母同源物的分离：人类同源物 (ORC5L) 映射到 7q22。
作者：
Ishiai,M;Dean,FB;Okumura,K;Abe,M;Moon,KY;Amin,AA;Kagotani,K;Taguchi,H;Murakami,Y;Hanaoka,F;O'Donnell,M;Hurwitz,J;Eki,T
Orc5p is a subunit of the origin recognition complex in the budding yeastSaccharomyces cerevisiae,which has been shown to play a critical role in both chromosomal DNA replication and transcriptional silencing. We have cloned cDNAs from both human and fission yeastSchizosaccharomyces pombethat encode proteins homologous to the budding yeast andDrosophilaOrc5p. Human Orc5p showed 35.1, 22.3, and 19.4% identity to theDrosophila, S. pombe,andS. cerevisiaeOrc5p, respectively. We have localized the human ORC5 gene (ORC5L) to chromosome 7 using Southern and PCR analysis of DNA isolated from a panel of human/rodent somatic cell hybrids and mapped the gene locus to 7q22 using fluorescencein situhybridization. We have identified a YAC clone that contains human ORC5L and maps to chromosome band 7q22.1. We have identified theS. pombeORC5 gene and located it in a cosmid mapped on chromosome II.
