EXPERIENTIAL INPUT ALTERS PHOSPHORYLATION OF SPECIFIC PROTEINS IN BRAIN MEMBRANES
EXPERIENTIAL INPUT ALTERS PHOSPHORYLATION OF SPECIFIC PROTEINS IN BRAIN MEMBRANES
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DOI：
10.1016/0091-3057(77)90068-5
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发表时间：
1977-01-01
影响因子：
3.6
通讯作者：
ROUTTENBERG, A
中科院分区：
文献类型：
--
作者：
EHRLICH, YH;RABJOHNS, RR;ROUTTENBERG, A
The effects of a training experience that involved foot shock on the endogenous phosphorylation of membrane-bound proteins from brain were studied. Crude membrane fractions were prepared from the cerebral cortex and neostriatum of animals that were sacrificed by quick freezing. In vitro incubation of the membranes with .gamma.-32P-ATP, followed by SDS[sodium dodecyl sulfate]-gel electrophoresis of the phosphorylated substrates, revealed that the phosphorylation of 2 protein components (designated F and H-I) increased in preparations from animals that were subjected to a training experience 24 h prior to sacrifice. These effects were greater in preparations from the neostriatum than from the cerebral cortex, and were observed in experiments using both rats and mice. Although all trained animals showed a high phosphorylation of bands F and H-I, control animals showed a greater variability in the phosphorylation of these bands. The phosphorylation of specific proteins may play a mediatory role in the processing of experiential information.
