Mechanical properties of membrane surface of cultured astrocyte revealed by atomic force microscopy
Mechanical properties of membrane surface of cultured astrocyte revealed by atomic force microscopy
复制标题
原子力显微镜揭示培养星形胶质细胞膜表面的机械特性
DOI：
10.1143/jjap.39.3711
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发表时间：
2000
影响因子：
1.5
通讯作者：
H. Haga
中科院分区：
文献类型：
--
作者：
H. Shiga;Y. Yamane;E. Ito;K. Abe;K. Kawabata;H. Haga
为了检测星形胶质细胞膜表面的力学性质，我们用原子力显微镜(AFM)在接触模式和作用力映射模式下观察了活的星形胶质细胞。在接触力模式下，在地形图上观察到了反映肌动蛋白细丝的脊状结构，而在力映射模式下，在零加载力下观察到了这种结构。当我们测量星形胶质细胞的弹性时，我们观察到细胞核上方的细胞膜柔软，胞浆上方的细胞膜僵硬。特别是，反映肌动蛋白细丝的部分非常坚硬。在应用肌动蛋白聚合抑制剂后，肌动蛋白细丝的丢失证实了肌动蛋白细丝对星形胶质细胞弹性的影响。
In order to examine the mechanical properties of the membrane surface of astrocytes, we observed living astrocytes by atomic force microscopy (AFM) both in contact mode and force-mapping mode. Ridge-like structures reflecting actin filaments were observed in the topographic images in contact mode, but not in force-mapping mode, using a zero-loading force. When we measured the elasticity of astrocytes, we observed that the cell membrane above the nucleus was soft and the cell membrane above the cytosol was stiff. In particular, the parts reflecting actin filaments were very stiff. This effect of actin filaments on the elasticity of astrocytes was confirmed by the loss of actin filaments after application of actin-polymerization inhibitor.