Characterization of ubiquitin ligase SlATL31 and proteomic analysis of 14-3-3 targets in tomato fruit tissue (Solanum lycopersicum L.)
Characterization of ubiquitin ligase SlATL31 and proteomic analysis of 14-3-3 targets in tomato fruit tissue (Solanum lycopersicum L.)
复制标题
DOI：
10.1016/j.jprot.2016.04.016
复制
发表时间：
2016-06-30
影响因子：
3.3
通讯作者：
Yamaguchi, Junji
中科院分区：
文献类型：
--
作者：
Lu, Yu;Yasuda, Shigetaka;Yamaguchi, Junji
14-3-3蛋白通过与磷酸化蛋白相互作用参与植物生理学的许多方面，从而调节靶蛋白的功能。在拟南芥中，泛素连接酶ATL 31通过直接相互作用和泛素化来控制14-3-3的稳定性，从而调节萌发后对碳和氮营养可用性的响应。由于14-3-3蛋白调节与营养代谢相关的许多关键酶的活性，因此可以预期它们不仅在营养组织中而且在生殖组织中发挥重要作用。由于果实产量在很大程度上取决于碳、氮的有效性及其利用，因此分析了番茄果实组织中14-3-3蛋白的功能。在这里，我们从番茄中分离并表征了泛素连接酶SIATL 31（Solyc 03 g112340），并证明SIATL 31具有泛素连接酶活性以及与番茄14-3-3蛋白的相互作用，这表明SIATL 31作为14-3-3的泛素连接酶的功能类似于其拟南芥直向同源物的可能性。此外，我们对14-3-3相互作用蛋白进行了蛋白质组学分析，并确定了106个蛋白质作为推定的14-3-3靶标，包括碳代谢和光合作用的关键酶。这一14-3-3相互作用组结果和可用的转录组图谱表明14-3-3蛋白在番茄果实组织中具有相当大而复杂的作用。生物学意义：大量累积证据表明14-3-3蛋白参与植物初级代谢的调节。在这里，我们提供了14-3-3相互作用组分析的第一份报告，并确定了番茄果实组织中假定的14-3-3靶点，这可能是非常重要的，因为记录的代谢变化，发生在果实发育和成熟过程中。这些数据开辟了未来的研究途径，了解番茄果实发育中的翻译后调控的作用。(C)© 2016 Elsevier B. V.版权所有。
The 14-3-3 proteins participate in many aspects of plant physiology by interacting with phosphorylated proteins and thereby regulating target protein functions. In Arabidopsis plant, the ubiquitin ligase ATL31 controls 14-3-3 stability via both direct interaction and ubiquitination, and this consequently regulates post-germinative growth in response to carbon and nitrogen nutrient availability. Since 14-3-3 proteins regulate the activities of many key enzymes related to nutrient metabolism, one would anticipate that they should play an essential role not only in vegetative but also in reproductive tissue. Because fruit yield largely depends on carbon and nitrogen availability and their utilization, the function of 14-3-3 proteins was analyzed in tomato fruit tissue. Here, we isolated and characterized an ubiquitin ligase SIATL31 (Solyc03g112340) from tomato and demonstrated that SIATL31 has ubiquitin ligase activity as well as interaction with tomato 14-3-3 proteins, suggesting the possibility that the SIATL31 functions as an ubiquitin ligase for 14-3-3 similarly to its Arabidopsis ortholog. Furthermore, we performed proteomic analysis of 14-3-3 interacting proteins and identified 106 proteins as putative 14-3-3 targets including key enzymes for carbon metabolism and photosynthesis. This 14-3-3 interactome result and available transcriptome profile suggest a considerable yet complex role of 14-3-3 proteins in tomato fruit tissue.Biological significance: Considerable cumulative evidence exists which implies that 14-3-3 proteins are involved in the regulation of plant primary metabolism. Here we provide the first report of 14-3-3 interactome analysis and identify putative 14-3-3 targets in tomato fruit tissue, which may be highly important given the documented metabolic shifts, which occur during fruit development and ripening. These data open future research avenues by which to understand the regulation of the role of post-translational regulation in tomato fruit development. (C) 2016 Elsevier B.V. All rights reserved.