PCR approach based on the esyn1 gene for the detection of potential enniatin-producing Fusarium species
PCR approach based on the esyn1 gene for the detection of potential enniatin-producing Fusarium species
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DOI：
10.1016/j.ijfoodmicro.2007.02.003
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发表时间：
2007-05-30
影响因子：
5.4
通讯作者：
Olszewski, Jacek
中科院分区：
文献类型：
--
作者：
Kulik, Tomasz;Pszczolkowska, Agnieszka;Olszewski, Jacek
镰刀菌头枯病（FHB）是由镰刀菌属的一系列真菌引起的小粒谷物和玉米的一种病害。该疾病降低种子的产量和质量，并导致各种真菌毒素的积累，这些毒素对人类和牲畜造成各种毒性影响。白僵菌素（Beauvericin，简称BAE）和恩镰孢菌素（Eniatins，简称ENs）是一类主要由F. avenaceum、燕麦F. poae和F.三带在这项研究中，引物组被设计为针对esyn 1基因同源物编码的多功能酶恩镰孢菌素合成酶。用于多重PCR的引物扩增了来自报告产生（EN）和/或EHF的FHB种属的产物。在源自北方和波兰南部的无症状小麦种子样品上开发的标记的使用证明，所有样品对于潜在的产生恩镰孢菌素的镰刀菌属物种的存在是阳性的。(c)2007 Elsevier B. V.保留所有权利。
Fusarium head blight (FHB) is a disease of small-grain cereals and com caused by a complex of fungal species of the genus Fusarium. The disease reduces the yield and quality of seeds and results in the accumulation of various mycotoxins which cause a variety of toxic effects on humans and livestock. Beauvericin (BEA) and enniatins (ENs) are a group of toxins with antimicrobial, insecticidal and phytotoxic activities produced mainly by F. avenaceum, F. poae and F. tricinctum. In this study, primer sets were designed that were targeted to esyn1 gene homologs encoding multifunctional enzyme enniatin synthetase. Primers used in multiplex PCR amplified products from the FHB species reported to produce (ENs) and/or BEA. The use of the marker developed on asymptomatic wheat seed samples originating from Northern and Southern Poland demonstrated that all samples were positive for the presence of potential enniatin-producing Fusarium species. (c) 2007 Elsevier B.V. All rights reserved.