Magnetite-Binding Flagellar Filaments Displaying the MamI Loop Motif
Magnetite-Binding Flagellar Filaments Displaying the MamI Loop Motif
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DOI：
10.1002/cbic.201600377
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发表时间：
2016-11-01
期刊：
影响因子：
3.2
通讯作者：
Vonderviszt, Ferenc
中科院分区：
文献类型：
--
作者：
Bereczk-Tompa, Eva;Posfai, Mihaly;Vonderviszt, Ferenc
这项工作旨在开发一种新的方法，利用突变的鞭毛丝来制备一维磁铁矿纳米结构。我们构建了四个显示磁铁结合基序的鞭毛蛋白突变体：其中两个含有趋磁细菌(Mami和Mms6)的磁小体相关蛋白片段，另外两个使用了合成序列。磁选择方法鉴定Mami突变体与磁铁矿具有最高的结合亲和力。从含有MAMI环的鞭毛蛋白亚基中构建的细丝被用作模板，通过从悬浮液中捕获它们来沿着细丝形成磁性纳米颗粒链。我们的研究提供了一个概念验证，即鞭毛细丝可以被设计成在环境条件下促进一维磁铁矿纳米结构的形成。此外，它还证明了Mami和磁铁矿之间的相互作用，并暗示了这种蛋白质在趋磁细菌中的作用。
This work aimed at developing a novel method for fabricating 1D magnetite nanostructures with the help of mutated flagellar filaments. We constructed four different flagellin mutants displaying magnetite-binding motifs: two contained fragments of magnetosome-associated proteins from magnetotactic bacteria (MamI and Mms6), and synthetic sequences were used for the other two. A magnetic selection method identified the MamI mutant as having the highest binding affinity to magnetite. Filaments built from MamI loop-containing flagellin subunits were used as templates to form chains of magnetite nanoparticles along the filament by capturing them from suspension. Our study represents a proof-of-concept that flagellar filaments can be engineered to facilitate formation of 1D magnetite nanostructures under ambient conditions. In addition, it proves the interaction between MamI and magnetite, with implications for the role of this protein in magnetotactic bacteria.