DNA introduction into living cells by water droplet impact with an electrospray process
DNA introduction into living cells by water droplet impact with an electrospray process
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DOI：
10.1002/anie.200704429
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发表时间：
2008-01-01
影响因子：
16.6
通讯作者：
Sakai, Takafumi
中科院分区：
文献类型：
--
作者：
Okubo, Yusuke;Ikemoto, Kazuto;Sakai, Takafumi
将白色来航受精卵在38℃下孵化，1.5天后培养出胚胎。将胚胎放入琼脂糖皿中，将含有2%坚固绿的GFP(4.3微克/毫升，1微升)放在胚胎的靶区。
White Leghorn fertile eggs were incubated at 38 C. After 1.5 days, an embryo was cultured. The embryo was placed in an agarose dish, and GFP plasmid (4.3 microg/mL, 1 microL) with 2% Fast Green was put on the target region of the embryo.