Nitric Oxide-Generating Antiplatelet Polyurethane Surfaces with Multiple Additional Biofunctions via Cyclodextrin-Based Host–Guest Interactions
Nitric Oxide-Generating Antiplatelet Polyurethane Surfaces with Multiple Additional Biofunctions via Cyclodextrin-Based Host–Guest Interactions
复制标题
通过基于环糊精的主客体相互作用产生一氧化氮的抗血小板聚氨酯表面具有多种附加生物功能
DOI：
10.1021/acsabm.9b00969
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发表时间：
2019
影响因子：
4.7
通讯作者：
Chen Hong
中科院分区：
文献类型：
--
作者：
Jin Sheng;Huang Jialei;Chen Xianshuang;Gu Hao;Li Dan;Zhang Aiyang;Liu Xiaoli;Chen Hong
通过亲水性单体甲基丙烯酸羟乙酯(HEMA)与共聚单体1-金刚烷基-1-甲基丙烯酸甲酯(ADAMA)的共聚反应，并加入硒半胱胺，制备了一种产生一氧化氮的聚合物涂料。将该涂料应用于聚氨酯(PU)基材。在NO供体存在的情况下，PU-PHA-Se表面生成一氧化氮(NO)。该表面可抑制血小板黏附和人脐静脉血管平滑肌细胞黏附和增殖。通过金刚烷基和环糊精(CD)衍生物之间的主客体相互作用，修饰表面上的聚(ADAMA)被设计成允许功能单元进入PU-PHA-Se表面。本工作在PU-PHA-Se表面引入了两种含赖氨酸(Cd-L)和磺酸基(Cd-S)的功能镉配合物。Cd-L具有纤溶活性，而Cd-S促进人脐静脉内皮细胞增殖。这种不生成抗血小板的聚合物涂层有可能成为通过主客体相互作用来修饰具有多种额外生物功能的表面的平台，从而为制备具有多功能的生物材料提供了一种替代方法。
A nitric oxide-generating polymeric coating was prepared by copolymerization of the hydrophilic monomer 2-hydroxyethyl methacrylate (HEMA) and the comonomer 1-adamantan-1-ylmethyl methacrylate (AdaMA) with subsequent incorporation of selenocystamine. The coating was applied to polyurethane (PU) as a substrate. In the presence of a NO donor, the PU–PHA-Se surface generated nitric oxide (NO). This surface was shown to inhibit platelet adhesion and human umbilical vein smooth muscle cell adhesion and proliferation. The poly(AdaMA) on the modified surface was designed to allow the incorporation of functional units into the PU–PHA-Se surface via host–guest interactions between the adamantane groups and cyclodextrin (CD) derivatives. In this work, two functional CD complexes containing lysine (CD-L) and sulfonate (CD-S) groups were incorporated into the PU–PHA-Se surface. CD-L conferred fibrinolytic activity, whereas CD-S promoted human umbilical vein endothelial cell proliferation. This NO-generating antiplatelet polymeric coating has potential as a platform for modifying surfaces with multiple additional biological functions via host–guest interactions, thus providing an alternative approach for the preparation of biomaterials with multifunctionality.