Prevention of biofilm formation and removal of existing biofilms by extracellular DNases of Campylobacter jejuni.
Prevention of biofilm formation and removal of existing biofilms by extracellular DNases of Campylobacter jejuni.
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DOI：
10.1371/journal.pone.0121680
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
van Vliet AH
中科院分区：
文献类型：
--
作者：
Brown HL;Reuter M;Hanman K;Betts RP;van Vliet AH
食源性细菌病原体空肠弯曲菌的挑剔性质与其在食物链中生存的能力形成对比。生物膜的形成，或整合到现有的生物膜C。空肠被认为有助于食物链的生存。由于细胞外DNA（eDNA）先前已被提出在C. jejuni生物被膜，我们研究了C.空肠生物膜形成。搜索2791 C。jejuni基因组中几乎一半的C.空肠弯曲菌基因组中至少含有一个eDNase基因，但只有少数分离株含有两个或三个eDNase基因，如C. jejuni菌株RM 1221，其含有cje0256、cje0566和cje1441 eDNase基因。菌株RM 1221不形成生物膜，而eDNase阴性菌株NCTC 11168和81116形成生物膜。NCTC 11168的预形成生物膜与活C.空肠RM1221或用来自RM1221培养物的用过的培养基培养导致生物膜的去除。在菌株RM1221中cje1441 eDNase基因的失活恢复了生物膜的形成，并且使突变体不能降解菌株NCTC 11168的生物膜。最后，对C. jejuni菌株RM1221能降解C.空肠NCTC 11168、81116和RM 1221菌株不能表达，而菌株NCTC 11168和RM 1221 cje1441突变体不能表达。这反映在C.空肠RM1221。这表明C.空肠炎影响生物膜形成并且不利于生物膜生活方式。然而，这些eDNase在控制C.食物链相关环境中的空肠菌株。
The fastidious nature of the foodborne bacterial pathogen Campylobacter jejuni contrasts with its ability to survive in the food chain. The formation of biofilms, or the integration into existing biofilms by C. jejuni, is thought to contribute to food chain survival. As extracellular DNA (eDNA) has previously been proposed to play a role in C. jejuni biofilms, we have investigated the role of extracellular DNases (eDNases) produced by C. jejuni in biofilm formation. A search of 2791 C. jejuni genomes highlighted that almost half of C. jejuni genomes contains at least one eDNase gene, but only a minority of isolates contains two or three of these eDNase genes, such as C. jejuni strain RM1221 which contains the cje0256, cje0566 and cje1441 eDNase genes. Strain RM1221 did not form biofilms, whereas the eDNase-negative strains NCTC 11168 and 81116 did. Incubation of pre-formed biofilms of NCTC 11168 with live C. jejuni RM1221 or with spent medium from a RM1221 culture resulted in removal of the biofilm. Inactivation of the cje1441 eDNase gene in strain RM1221 restored biofilm formation, and made the mutant unable to degrade biofilms of strain NCTC 11168. Finally, C. jejuni strain RM1221 was able to degrade genomic DNA from C. jejuni NCTC 11168, 81116 and RM1221, whereas strain NCTC 11168 and the RM1221 cje1441 mutant were unable to do so. This was mirrored by an absence of eDNA in overnight cultures of C. jejuni RM1221. This suggests that the activity of eDNases in C. jejuni affects biofilm formation and is not conducive to a biofilm lifestyle. These eDNases do however have a potential role in controlling biofilm formation by C. jejuni strains in food chain relevant environments.