TNFα promotes proliferation of human synovial MSCs while maintaining chondrogenic potential.
TNFα promotes proliferation of human synovial MSCs while maintaining chondrogenic potential.
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DOI：
10.1371/journal.pone.0177771
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Sekiya I
中科院分区：
文献类型：
--
作者：
Shioda M;Muneta T;Tsuji K;Mizuno M;Komori K;Koga H;Sekiya I
滑膜间充质干细胞（MSC）是软骨和半月板再生的候选细胞来源。如果我们能更有效地增殖滑膜间充质干细胞，我们可以扩大临床应用到大的软骨和半月板病变的患者。TNFα是一种多效性细胞因子，可影响体内细胞的生长和分化。本研究的目的是检测TNFα对人滑膜间充质干细胞增殖、软骨形成和其他特性的影响。取2名供体的第1代人滑膜间充质干细胞，加入2.5 × 10−12~10−7 μ g/ml、10倍稀释系列的TNFα培养14天，计数细胞数和集落数。在来自6个供体的滑膜MSC中还检查了最佳剂量的TNFα对增殖的影响。用TNFα预处理的滑膜间充质干细胞在6名供体中评估软骨形成潜力。3名供者12种表面抗原的表达也被检测到，2.5ng/ml和更高浓度的TNFα显著增加了两名供者的细胞数/皿和细胞数/集落。25 ng/ml TNFα的作用在所有6名供体中得到证实。未处理的MSC和用25 ng/ml TNFα预处理的MSC之间，软骨颗粒的重量、糖胺聚糖和DNA含量没有显着差异。TNFα降低3例供者CD 105和140 b表达率。TNFα促进滑膜MSCs增殖，细胞数/集落数增加。TNFα预处理不影响滑膜间充质干细胞的软骨形成。但TNFα对滑膜间充质干细胞的某些特性有影响。
Synovial mesenchymal stem cells (MSCs) are a candidate cell source for cartilage and meniscus regeneration. If we can proliferate synovial MSCs more effectively, we can expand clinical applications to patients with large cartilage and meniscus lesions. TNFα is a pleiotropic cytokine that can affect the growth and differentiation of cells in the body. The purpose of this study was to examine the effect of TNFα on proliferation, chondrogenesis, and other properties of human synovial MSCs. Passage 1 human synovial MSCs from 2 donors were cultured with 2.5 x 10−12~10−7 g/ml, 10 fold dilution series of TNFα for 14 days, then the cell number and colony number was counted. The effect of the optimum dose of TNFα on proliferation was also examined in synovial MSCs from 6 donors. Chondrogenic potential of synovial MSCs pretreated with TNFα was evaluated in 6 donors. The expressions of 12 surface antigens were also examined in 3 donors.2.5 ng/ml and higher concentration of TNFα significantly increased cell number/dish and cell number/colony in both donors. The effect of 25 ng/ml TNFα was confirmed in all 6 donors. There was no significant difference in the weight, or amount of glycosaminoglycan and DNA of the cartilage pellets between the MSCs untreated and MSCs pretreated with 25 ng/ml TNFα. TNFα decreased expression rate of CD 105 and 140b in all 3 donors. TNFα promoted proliferation of synovial MSCs with increase of cell number/ colony. Pretreatment with TNFα did not affect chondrogenesis of synovial MSCs. However, TNFα affected some properties of synovial MSCs.