Chemical interactions of mercury species and some transition and noble metals towards metallothionein (Zn7MT-2) evaluated using SEC/ICP-MS, RP-HPLC/ESI-MS and MALDI-TOF-MS.
Chemical interactions of mercury species and some transition and noble metals towards metallothionein (Zn7MT-2) evaluated using SEC/ICP-MS, RP-HPLC/ESI-MS and MALDI-TOF-MS.
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DOI：
10.1039/c3mt00016h
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发表时间：
2013-06
期刊：
影响因子：
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通讯作者：
Ming Xu;Limin Yang;Qiuquan Wang
中科院分区：
文献类型：
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作者：
Ming Xu;Limin Yang;Qiuquan Wang
用SEC/ICPMS、RP-HPLC/ESI-IT-MS和MALDI-TOF-MS研究了生理溶液环境中三种亚金属(“Mn、Co、Ni、Cu、Cd”、“Pd、Pt、Au”和“Hg、CH3Hg、C2H5Hg-Thi”)对金属硫蛋白-2(Zn7MT-2)的化学亲和力，其顺序为：“Zn(2+)Ni(2+)>；Mn(2+)，Co(2+)”；“Pd(2+)>；Au(3+)>；Pt(2+)“和”Hg(2+)>；CH3Hg(+)>；C2H5Hg-Thi“。此外，用CD光谱、RP-HPLC/ESI-MS和MALDI-TOF-MS研究了CH3Hg(+)滴定过程中生成的CH3Hg-MT-2络合物的结构变化和组成，结果表明(CH3Hg)XMT-2(x=12，13，14，15，16，17，18，19和20)形成了线形的疏水性更强的(CH3Hg)XMT-2(x=12，13，14，15，16，17，18，19和20)。这一信息对于了解金属与锌的相互作用及其相应的生物功能和毒性是很重要的。
The chemical affinities of three submetallomes ("Mn, Co, Ni, Cu, Cd"; "Pd, Pt, Au"; and "Hg, CH3Hg, C2H5Hg-THI") towards metallothionein-2 (Zn7MT-2) in a physiological solution environment were evaluated using SEC/ICP-MS together with RP-HPLC/ESI-IT-MS and MALDI-TOF-MS, and followed the order: "Zn(2+) Ni(2+) > Mn(2+), Co(2+)"; "Pd(2+) > Au(3+) > Pt(2+)"; and "Hg(2+) > CH3Hg(+) > C2H5Hg-THI". Besides these, the structural change and composition of the CH3Hg-MT-2 complexes formed during the CH3Hg(+) titration process were further investigated using CD spectroscopy, RP-HPLC/ESI-MS and MALDI-TOF-MS, indicating that linear and more hydrophobic (CH3Hg)xMT-2 (x = 12, 13, 14, 15, 16, 17, 18, 19 and 20) were formed. This information is important in understanding the interactions of the metals with Zn7MT-2 and their corresponding biological functions and toxicities.