Sequence and expression divergence of the AOC gene family in soybean: insights into functional diversity for stress responses
Sequence and expression divergence of the AOC gene family in soybean: insights into functional diversity for stress responses
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DOI：
10.1007/s10529-011-0585-9
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发表时间：
2011-04
影响因子：
2.7
通讯作者：
Qian Wu;Juanjuan Wu;Huai-Yong Sun;Dan Zhang;Deyue Yu
中科院分区：
文献类型：
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作者：
Qian Wu;Juanjuan Wu;Huai-Yong Sun;Dan Zhang;Deyue Yu
茉莉酸作为一种信号分子，在植物对各种生物和非生物胁迫的防御反应中起着至关重要的作用。丙二烯氧化物环化酶（AOC：EC5.3.99.6）催化茉莉酸生物合成途径中最重要的步骤。从大豆中分离到6个AOC基因，随机分布在第1、2、8、13、18和19染色体上。6个AOC蛋白质聚类为3类，相似性值在55 - 95%之间。实时PCR显示AOC基因在多个器官和多种胁迫下具有特异且复杂的表达模式。在转基因烟草中过量表达GmAOC 1和GmAOC 5基因分别增强了对盐胁迫和氧化胁迫的耐受性。AOC基因家族内如此大的多样性可能是大豆基因组进化过程中形成的一种适应机制。
As a signaling molecule, jasmonate plays a crucial role in orchestrating plant defense responses to a variety of biotic and abiotic stresses. Allene oxide cyclase (AOC: EC5.3.99.6) catalyzes the most important step in the jasmonate biosynthesis pathway. SixAOCgenes were isolated from soybean, randomly distributed on chromosomes 1, 2, 8, 13, 18 and 19. The six AOC proteins were clustered into three groups with similarity values ranging from 55 to 95%. Real-time PCR revealed that theAOCgenes have specific and complex expression patterns in multiple organs and under several stresses. Overexpression ofGmAOC1andGmAOC5gene in transgenic tobacco, respectively enhanced tolerance to salinity and oxidative stresses. Such a large diversity within theAOCgene family might be an adaptive mechanism that developed during soybean genome evolution.