CHANGES IN PLASMA HYPOXANTHINE AND FREE-RADICAL MARKERS DURING EXERCISE IN MAN
CHANGES IN PLASMA HYPOXANTHINE AND FREE-RADICAL MARKERS DURING EXERCISE IN MAN
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DOI：
10.1111/j.1748-1716.1991.tb09157.x
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发表时间：
1991-06-01
期刊：
影响因子：
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通讯作者：
CIZINSKY, S
中科院分区：
文献类型：
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作者：
SAHLIN, K;EKBERG, K;CIZINSKY, S
8名男性在最大摄氧量的44%和72%的负荷下骑行约6分钟，在98%的最大摄氧量下疲劳。分别在休息和运动时采集臂动脉和股静脉的血样。血浆次黄嘌呤、黄嘌呤和尿酸在疲劳时和恢复10分钟后显著升高。只有次黄嘌呤显示出显著的动-股静脉差异。从腿部释放的次黄嘌呤在恢复期增加，运动后10分钟是运动结束时的三倍。结论：大强度运动时血浆次黄嘌呤的显着升高来源于工作肌肉，而向黄嘌呤和尿酸盐的转化可能发生在其他组织中。以谷胱甘肽、高铁血红蛋白和丙二醛(MDA)作为血浆自由基标志物。在大强度运动中，血液和血浆中的总谷胱甘肽(谷胱甘肽+谷胱甘肽二硫)含量增加，这可能是自由基形成的迹象。然而，在任何情况下都不能在血浆中检测到丙二醛。1-MU-摩尔×L-1血浆)和高铁血红蛋白在运动中略有下降。需要使用更具体的技术进行进一步研究，以确定在短暂的高强度运动后，自由基的形成是否会增加。
Eight men cycled for about 6 minutes at workloads corresponding to 44 and 72% of maximal oxygen uptake and to fatigue at 98% maximal oxygen uptake. Blood samples from a brachial artery and a femoral vein were taken at rest and during exercise. Hypoxanthine, xanthine and urate in plasma were significantly elevated at fatigue and after 10 minutes of recovery. Only hypoxanthine showed a significant arterio-femoral venous difference. The release of hypoxanthine from the legs increased during the recovery period and was three-fold higher 10 minutes post exercise than at the end of exercise. It is concluded that the marked increase in plasma hypoxanthine which occurs during intensive exercise originates from the working muscle whereas the transformation to xanthine and urate may occur in other tissues. Glutathione, methemoglobin and malondialdehyde (MDA) were used as plasma markers of free radicals. Total glutathione (glutathione + glutathionedisulfide) in blood and plasma increased during intensive exercise and may be indicative of free radical formation. However, MDA was not detectable in plasma during any conditions (< 0. 1-mu-mol x l-1 plasma) and methemoglobin decreased slightly during exercise. Further studies using more specific techniques are required to determine whether the formation of free radicals is increased after brief intensive exercise.