In vivo estimation of tracheal wall water: effects of histamine and carbachol.
In vivo estimation of tracheal wall water: effects of histamine and carbachol.
复制标题
气管壁水的体内估计：组胺和卡巴胆碱的影响。
DOI：
10.1152/jappl.1986.60.5.1680
复制
发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Wanner,A
中科院分区：
文献类型：
--
作者：
Baier,H;Moas,R;Yerger,L;Wanner,A
基于双指示剂稀释原理，我们开发并验证了一种通过用惰性气体氦气和水溶性气体二甲醚绘制时间-浓度曲线来测量气管壁含水量的新方法。在有意识的成年羊的气管中，15 厘米长度内壁的可测量水量在 0.50 至 0.70 毫升之间。根据氦气量和已知气管长度计算出的气管半径在 7.4 至 9.3 毫米之间。相应的可测量“水深”范围在 63 至 82 微米之间。支气管血流量的变化对气管水量没有一致的影响。静脉注射组胺（4微克/千克）将平均水深增加至基线的122%至134%（P小于0.001），而静脉注射卡巴胆碱（4微克/千克）对水深没有影响。在绘制指示剂稀释曲线之前，除去由卡巴胆碱产生的大量气道分泌物。这些发现表明，双气体指示器技术能够在体内测量气管壁中水室的体积，并且该体积会因组胺而增加。该方法可能适用于人类受试者。
Based on double-indicator-dilution principles we developed and validated a new method of measuring water content in the tracheal wall by inscribing time-concentration curves with the inert gas, helium and the water-soluble gas, dimethylether. In the trachea of conscious adult sheep the measurable water volume of the wall over a 15-cm length ranged between 0.50 and 0.70 ml. The tracheal radius calculated from helium volume and known tracheal length ranged between 7.4 and 9.3 mm. The corresponding measurable “water depth” ranged between 63 and 82 microns. Changes in bronchial blood flow had no consistent influence on tracheal water volume. The intravenous injection of histamine (4 micrograms/kg) increased mean water depth to between 122 and 134% of base line (P less than 0.001), whereas the intravenous injection of carbachol (4 micrograms/kg) had no effect on water depth. The production of large amounts of airway secretions produced by carbachol were removed prior to inscription of the indicator-dilution curves. These findings indicate that the double-gas-indicator technique is capable of measuring in vivo the volume of a water compartment in the tracheal wall and that this volume is increased by histamine. This method may be applicable in human subjects.