Changes in rhinovirus protein 2C allow efficient replication in mouse cells
Changes in rhinovirus protein 2C allow efficient replication in mouse cells
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DOI：
10.1128/jvi.77.8.4773-4780.2003
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发表时间：
2003-04-01
影响因子：
5.4
通讯作者：
Racaniello, VR
中科院分区：
文献类型：
--
作者：
Harris, JR;Racaniello, VR
16型鼻病毒被发现在表达病毒受体人细胞间黏附分子1的小鼠L细胞中复制。然而，在低感染复数的情况下感染这些细胞导致没有明显的细胞病变效应，并且产生低病毒滴度。鼻病毒16株在HeLa和ICAM-1 L细胞间交替传代，分离到变异病毒16/L。16/L感染小鼠细胞后，病毒滴度升高，核糖核酸产生增加，细胞总病变效应增强。在病毒16/L的P2区有三个氨基酸变化，适应表型对应于蛋白2C的两个变化。鼻病毒宿主范围突变的特征将有助于研究病毒有效生长所需的细胞蛋白，并发展鼻病毒感染的小鼠模型。
Rhinovirus type 16 was found to replicate in mouse L cells that express the viral receptor, human intercellular adhesion molecule 1 (ICAM-1). However, infection of these cells at a low multiplicity of infection leads to no discernible cytopathic effect, and low virus titers are produced. A variant virus, 16/L, was isolated after alternate passage of rhinovirus 16 between HeLa and ICAM-1 L cells. Infection of mouse cells with 16/L leads to higher virus titers, increased production of RNA, and total cytopathic effect. Three amino acid changes were identified in the P2 region of virus 16/L, and the adaptation phenotype mapped to two changes in protein 2C. The characterization of a rhinovirus host range mutant will facilitate the investigation of cellular proteins required for efficient viral growth and the development of a murine model for rhinovirus infection.