Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA from India
Population structure of the rice sheath blight pathogen Rhizoctonia solani AG-1 IA from India
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DOI：
10.1007/s10658-005-1753-3
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发表时间：
2005-06-01
影响因子：
1.8
通讯作者：
Gnanamanickam, SS
中科院分区：
文献类型：
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作者：
Linde, CC;Zala, M;Gnanamanickam, SS
利用7个RFLP位点对印度水稻纹枯病菌RhizoctoniasolaniAG-1 IA的96个分离物的群体结构进行了分析。其中19株不与R. solani AG-1 IA RFLP探针和rDNA分析证实它们是水稻长梗霉（Ceratobasidium oryzae-sativae）分离物或另一种丝核菌（Rhizoctonia sp.）solani AG-1 IA印度分离物与先前表征的德克萨斯种群的分离物相似。R.印度的Solani AG-1 IA在田间表现中等，田间种群之间没有共享克隆。低水平的种群细分和种群间的遗传距离小是一致的高水平的基因流。大多数种群处于Hardy-Weinberg平衡（HWE），表明有性生殖频繁。两个位点（R68和R111），偏离HWE显着表现出过度的杂合性。虽然得克萨斯州和印度人口在地理上非常遥远，但他们只表现出适度的人口细分，F-ST值为0.193。
The population structure of Rhizoctonia solani AG-1 IA causing rice sheath blight from India was evaluated for 96 isolates using seven RFLP loci. Nineteen of the isolates did not hybridise to R. solani AG-1 IA RFLP probes and rDNA analyses subsequently confirmed that they were either Ceratobasidium oryzae-sativae isolates or another Rhizoctonia sp. The population structure of the remaining 77 R. solani AG-1 IA Indian isolates was similar to that of a previously characterized Texas population. Clonal dispersal of R. solani AG-1 IA in India was moderate within fields and no clones were shared among field populations. Low levels of population subdivision and small genetic distances among populations were consistent with high levels of gene flow. Frequent sexual reproduction was indicated by the fact that most populations were in Hardy-Weinberg equilibrium (HWE). The two loci (R68 and R111) that deviated significantly from HWE showed an excess of heterozygosity. Although Texas and Indian populations were geographically very distant, they exhibited only moderate population subdivision, with an F-ST value of 0.193.