Developmental origin of the Pair1 descending interneuron.
Developmental origin of the Pair1 descending interneuron.
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DOI：
10.17912/micropub.biology.000707
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发表时间：
2022
影响因子：
--
通讯作者：
Lee, Kristen
中科院分区：
文献类型：
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作者：
Linskens, Amanda;Doe, Chris;Lee, Kristen
Pair1是果蝇幼虫运动回路的一部分，通过抑制向前运动来促进向后运动。我们假设，谱系相关的神经元可能在神经元回路中共同发挥作用。验证这一假设需要知道运动回路中每个神经元的祖细胞，这里我们只关注Pair1。在果蝇胚胎发育过程中，独特的神经母细胞通过遗传其出生部位神经外胚层中表达的空间转录因子(TF)而形成。我们使用免疫荧光来检查Pair1神经元，以确定Pair1来自哪个神经母细胞。我们的结果表明，Pair1来源于表达猕猴桃(GSB)和中间神经母细胞缺陷(IND)的下颌神经母细胞5-3。当GSB或Ind在Pair1谱系中过表达时，额外的神经元形成具有相似Pair1形态的额外神经元。
Pair1 is part of a Drosophila larval locomotor circuit that promotes backward locomotion by inhibiting forward locomotion. We hypothesize that lineage related neurons may function in neuronal circuits together. Testing this hypothesis requires knowing the progenitor of each neuron within this locomotor circuit, and here we focus exclusively on Pair1. During Drosophila melanogaster embryogenesis, unique neuroblasts form by inheriting the spatial transcription factors (TFs) expressed in their birth location within the neuroectoderm. We examine the Pair1 neurons using immunofluorescence to determine which neuroblast the Pair1s derive from. Our results show that Pair1 is derived from gnathal neuroblast 5-3 which expresses Gooseberry (Gsb) and Intermediate neuroblasts defective (Ind). When Gsb or Ind were overexpressed in the Pair1 lineage, extra neurons formed with similar Pair1 morphology.