Folding and Imaging of DNA Nanostructures in Anhydrous and Hydrated Deep-Eutectic Solvents
Folding and Imaging of DNA Nanostructures in Anhydrous and Hydrated Deep-Eutectic Solvents
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DOI：
10.1002/anie.201412354
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发表时间：
2015-06-01
影响因子：
16.6
通讯作者：
Hud, Nicholas V.
中科院分区：
文献类型：
--
作者：
Gallego, Isaac;Grover, Martha A.;Hud, Nicholas V.
人们对DNA纳米技术非常感兴趣，但它的应用仅限于含水或大量水合的介质。现在描述了DNA纳米结构在无水溶剂中的首次组装，即由甘油和氯化胆碱(甘胆碱)的4：1混合物组成的低挥发性生物相容深共晶溶剂。甘胆碱可以在20摄氏度下在6天内折叠二维DNA折纸，而在水合甘胆碱中，折叠速度加快(3小时)。此外，在等温条件下，三维DNA折纸和DNA尾部系统可以在水合甘胆碱中折叠。甘胆碱明显减少了在水溶液中折叠时遇到的动力学陷阱。此外，折叠结构可以在水溶液和甘胆碱之间转移。预计甘胆碱和类似的溶剂将允许通过提供具有可调节的性质的环境来创建更复杂的功能DNA结构，该环境可以针对一系列应用和纳米结构进行优化。
There is great interest in DNA nanotechnology, but its use has been limited to aqueous or substantially hydrated media. The first assembly of a DNA nanostructure in a water-free solvent, namely a low-volatility biocompatible deep-eutectic solvent composed of a 4:1 mixture of glycerol and choline chloride (glycholine), is now described. Glycholine allows for the folding of a two-dimensional DNA origami at 20 degrees C in six days, whereas in hydrated glycholine, folding is accelerated (3h). Moreover, a three-dimensional DNA origami and a DNA tail system can be folded in hydrated glycholine under isothermal conditions. Glycholine apparently reduces the kinetic traps encountered during folding in aqueous solvent. Furthermore, folded structures can be transferred between aqueous solvent and glycholine. It is anticipated that glycholine and similar solvents will allow for the creation of functional DNA structures of greater complexity by providing a milieu with tunable properties that can be optimized for a range of applications and nanostructures.