Intestinal mucins inhibit rotavirus replication in an oligosaccharide-dependent manner.
Intestinal mucins inhibit rotavirus replication in an oligosaccharide-dependent manner.
复制标题
肠道粘蛋白以寡糖依赖性方式抑制轮状病毒复制。
DOI：
10.1093/infdis/169.5.1002
复制
发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Forstner,JF
中科院分区：
文献类型：
--
作者：
Yolken,RH;Ojeh,C;Khatri,IA;Sajjan,U;Forstner,JF
轮状病毒是全球婴儿发病和死亡的重要原因。先前已经表明，粘液糖蛋白可以抑制轮状病毒复制。然而，这种抑制作用的结构与功能关系尚未完全阐明。用CsCl密度梯度超离心法从大鼠和人小肠上皮刮片中纯化粘蛋白，并在MA-104细胞中测试其对轮状病毒复制的抑制作用。天然人和大鼠肠粘蛋白在低浓度下抑制人和动物轮状病毒的复制。抗病毒活性在大鼠和人粘蛋白的密集糖基化部分中最为突出。硫醇还原和烷基化，氯仿甲醇分配，并部分去除寡糖后，活性保留。然而，粘蛋白的总去糖基化破坏了抗病毒活性。来自人类和其他动物的肠粘蛋白是轮状病毒复制的有效抑制剂，并且这种抑制依赖于特定的粘蛋白-病毒相互作用。
Rotaviruses are important causes of infant morbidity and mortality worldwide. It has been previously shown that mucinous glycoproteins can inhibit rotavirus replication. However, the structure-function relationships of this inhibition have not been completely elucidated. Mucins were purified from epithelial scrapings of rat and human intestine by CsCI density-gradient ultracentrifugation and tested for the inhibition of rotavirus replication in MA-I04 cells. Native human and rat intestinal mucins inhibited the replication of human and animal rotaviruses at low concentrations. Antiviral activity was most prominent in the densely glycosylated part of the rat and human mucins. Activity was retained after thiol reduction and alkylation, chloroform methanol partition, and partial removal of oligosaccharides. However, total deglycosylation of the mucins destroyed antiviral activity. Intestinal mucins from humans and other animals are potent inhibitors of rotavirus replication, and this inhibition is dependent on specific mucin-viral interactions.