Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells.
Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells.
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亲代腺病毒 DNA 在受感染细胞的核小体样结构中积累。
DOI：
10.1093/nar/6.8.2769
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发表时间：
1979
影响因子：
14.9
通讯作者：
L. Philipson
中科院分区：
文献类型：
--
作者：
V. Tate;L. Philipson
微球菌核酸酶酶切腺病毒2（ad2）感染后早期的HeLa细胞核已被用于研究亲本病毒DNA的核蛋白性质。病毒DNA比细胞DNA更容易被核酸酶消化。消化产物的模式随着消化从感染后1小时（pi）的模糊模式到6小时（pi）的核小体样模式的变化而变化。病毒和细胞核蛋白产品之间的主要区别是：1)病毒DNA的亚核小体部分；2)病毒核小体中的DNA重复长度为165个碱基对，细胞核小体中的DNA重复长度为195个碱基对。细胞核中高达50%的病毒DNA似乎是核小体样结构。在部分未包被的病毒或分离的病毒核的消化过程中看不到这种模式。
Micrococcal-nuclease digestion of adenovirus 2(ad 2) infected HeLa cell nuclei early after infection has been used to investigate the nucleoprotein nature of parental viral DNA. Viral DNA is more susceptible to nuclease digestion than cellular DNA. The pattern of digestion products changes as digestion proceeds from an indistinct pattern 1 hour post infection(pi) to a nucleosome-like pattern at 6 hours pi. The major differences between viral and cellular nucleoprotein products were i) a subnucleosome fraction from viral DNA and ii) the repeat size of DNA in viral nucleosomes was 165 base pairs and in cellular nucleosomes, 195 base pairs. Up to 50% viral DNA in nuclei 6 hours pi seems to be in nucleosome-like structures. Such patterns are not seen on digestion of partially-uncoated virus or isolated cores.