pH-sensitive photoluminescence of CdSe/ZnSe/ZnS quantum dots in human ovarian cancer cells
pH-sensitive photoluminescence of CdSe/ZnSe/ZnS quantum dots in human ovarian cancer cells
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DOI：
10.1021/jp0654718
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发表时间：
2007-02-22
影响因子：
3.7
通讯作者：
Gundersen, Martin A.
中科院分区：
文献类型：
--
作者：
Liu, Yu-San;Sun, Yinghua;Gundersen, Martin A.
在SKOV-3人卵巢癌细胞中，硫代乙酸(MAA)修饰的半导体纳米晶量子点(QDS)的光致发光是pH依赖的，这表明量子点作为细胞内pH传感器的应用。在固定细胞和活细胞中，细胞内MAA修饰的量子点(MAA量子点)的荧光强度随着pH的升高而单调增加。MAA量子点的电泳率也随着pH的增加而增加，表明表面电荷和荧光发射之间存在联系。在低pH下，MAA从硫化锌外壳解离，导致聚集和溶解性丧失，这也可能是在细胞内环境中观察到的MAA量子点荧光变化的原因。
The photoluminescence of mercaptoacetic acid (MAA)-capped CdSe/ZnSe/ZnS semiconductor nanocrystal quantum dots (QDs) in SKOV-3 human ovarian cancer cells is pH-dependent, suggesting applications in which QDs serve as intracellular pH sensors. In both fixed and living cells the fluorescence intensity of intracellular MAA-capped QDs (MAA QDs) increases monotonically with increasing pH. The electrophoretic mobility of MAA QDs also increases with pH, indicating an association between surface charging and fluorescence emission. MAA dissociates from the ZnS outer shell at low pH, resulting in aggregation and loss of solubility, and this may also contribute to the MAA QD fluorescence changes observed in the intracellular environment.